文献类型：期刊文献

英文题名：Screening differentially expressed genes and the pathogenesis in atopic dermatitis using bioinformatics

作者：Yang, Pengfei[1];Li, Yanmei[1];Li, Tingbao[1];Yuan, Liang[1];Wang, Sinong[1]

第一作者：杨鹏斐

通信作者：Wang, SN[1]

机构：[1]Gansu Univ Chinese Med, Clin Coll Chinese Med, Lanzhou 730000, Gansu, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Clin Coll Chinese Med, Lanzhou 730000, Gansu, Peoples R China.|[10735]甘肃中医药大学;

年份：2023

卷号：69

期号：15

起止页码：73

外文期刊名：CELLULAR AND MOLECULAR BIOLOGY

收录：;Scopus(收录号：2-s2.0-85183507732);WOS:【SCI-EXPANDED(收录号:WOS:001185716600012)】；

语种：英文

外文关键词：AD; bioinformatics; differential expression; genes; pathogenesis; inflammatory response

摘要：Atopic dermatitis (AD) is a common chronic skin inflammation. It was to screen differentially expressed genes (DEGs) and related biological functional pathways in atopic dermatitis (AD) by bioinformatics methods, and to understand the pathogenesis of AD. gene chip datasets GSE120721 and GSE32924 in the public database NCBI Gene Expression Omnibus (GEO) were adopted. Differential expression analysis between the patient group and controls was performed by applying the zero-code differential expression analysis tool GEO2R, and a few DEGs were screened. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were carried out. In addition, the STRING online database was employed to predict the potential relationship among DEGs, the protein-protein interaction network (PPI) was drawn, and the module analysis of PPI was performed using the Cytoscape plugin MCODE. 233 DEGs were screened out, including 134 up-regulated genes and 99 down-regulated genes. GO analysis suggested that these DEGs were mainly involved in biological processes (BP), cellular components (CC), and molecular function (MF). KEGG analysis displayed that these DEGs were mainly involved in NF-kappa B signaling, cell cycle, T cell receptor signaling, and other pathways. PPI analysis indicated that there were complex interactions among DEGs, and module analysis further revealed the important roles of DEGs in regulating immune response, inflammatory response, and skin barrier function. The above findings provide a valuable reference for the development of new treatment options.