文献类型：期刊文献

中文题名：烘制法对党参中党参炔苷含量的影响

英文题名：Effects of baking method on the contents of Lobetyolin in Codonopsis pilosula

作者：李越峰[1];张泽国[1];徐富菊;吴平安[1];刘峰林[1];金辉[1];严兴科[1]

第一作者：李越峰

机构：[1]甘肃中医学院,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2015

卷号：34

期号：2

起止页码：38

中文期刊名：中兽医医药杂志

外文期刊名：Journal of Traditional Chinese Veterinary Medicine

基金：国家自然科学基金(81073077);教育部科学技术研究重点项目(212186);甘肃省财政厅高校基本科研业务费专项资金(2013-2);2010年度甘肃省自然科学基金(1010RJZA212);2010甘肃中医学院引进人才科研启动基金(2010-3)

语种：中文

中文关键词：党参;蜜炙党参;烘制法;党参炔苷;HPLC

外文关键词：Codonopsis pilosula;;honey-processed Codonopsis pilosula;;baking processed;;Lobetyolin;;HPLC

摘要：目的:旨在测定不同烘制温度下党参炮制品中党参炔苷的含量,为科学评价及有效控制其质量提供可靠方法。方法:采用HPLC法、梯度洗脱及超声提取法。色谱条件为:色谱柱:Kromasil C18色谱柱(4.6 mm×250 mm,5μm);流速:1.0 m L/min;检测波长:党参炔苷267 nm,柱温:25℃;流动相:乙腈-0.05%磷酸梯度洗脱系统。结果:通过对比分析不同烘制温度下得到的炮制品,测定条件相同时,烘制党参中党参炔苷的含量高于生品;烘制温度为40℃时,蜜炙党参中的党参炔苷含量较高,烘制温度与党参中党参炔苷的含量无线性关系;蜜炙党参自然干燥品中党参炔苷含量较低。结论:不同烘制温度对党参中党参炔苷含量有一定影响;蜜炙党参最佳烘制温度为40℃。同时,采用HPLC方法测定党参中党参炔苷的含量,操作简单,结果可靠,重现性好,可作为甘肃道地药材党参及其炮制品质量控制的有效方法。
Objective: To determine the content of Lobetyolin in processed Codonopsis pilosula products under different baking temperature, and provide reliable methods for its scientific evaluation and quality control. Methods: HPLC, gradient elution and ultrasonic extraction method were used. The chromatographic conditions were as follows: the chromatographic column: Kromasil C18 chromatographic column(4.6 mm×250 mm, 5 μm), flow rate: 1.0 m L/min, detection wavelength: 267 nm,column temperature: 25 ℃, the mobile phase: acetonitrile-0.05% phosphoric acid water gradient elution system. Results:Under same conditions, the honey-processed Codonopsis pilosula products present higher Lobetyolin content than crude products, especially at the baking temperature of content of 40 ℃. There was no linearity between baking temperature and Lobetyolin content in processed Codonopsis pilosula. Conclusion: Different baking temperature can affect Lobetyolin content in processed Codonopsis pilosula. Honey-processed Codonopsis pilosula should be best to bake at 40 ℃. HPLC could be used for effectively quality control of processed Codonopsis pilosula products with simple operation, reliable results and good repeatability.