文献类型：期刊文献

英文题名：A rapid response "Turn-On" fluorescent probe for nitroreductase detection and its application in hypoxic tumor cell imaging

作者：Xu, Jian[1,2,3];Sun, Shaobo[4];Li, Qian[1,2];Yue, Ying[1,2];Li, Yingdong[4];Shao, Shijun[1,2]

第一作者：Xu, Jian

通信作者：Li, YD[1]

机构：[1]Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China;[2]Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China;[3]Univ Chinese Acad Sci, Beijing 100039, Peoples R China;[4]Gansu Univ Chinese Med, Inst Integrated Tradit & Western Med, Lanzhou 730000, Peoples R China

第一机构：Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Inst Integrated Tradit & Western Med, Lanzhou 730000, Peoples R China.|[10735ed249c6606940a33]甘肃中医药大学中西医结合学院;[10735]甘肃中医药大学;

年份：2015

卷号：140

期号：2

起止页码：574

外文期刊名：ANALYST

收录：;Scopus(收录号：2-s2.0-84918545365);WOS:【SCI-EXPANDED(收录号:WOS:000346304200023)】；

基金：This work was supported by the National Natural Science Foundation of China (20972170 and 21275150), the Funds for Distinguished Young Scientists of Gansu (1210RJDA013) and the Natural Science Foundation of Gansu province (1107RJYA069).

语种：英文

摘要：A novel "Turn-On" fluorescent probe, quaternarized 4-pyridinyl-substituted BODIPY dye by incorporating a 5-nitrofuran moiety, was developed and applied for imaging the hypoxic status of tumor cells by the indirect detection of nitroreductase. The design was based on a nitroreductase-catalyzed reduction of the nitrofuran moiety in the presence of reduced nicotinamide adenine dinucleotide (NADH) as an electron donor and followed by the 1,6-rearrangement-elimination and the release of free 4-pyridinyl-substituted BODIPY dye 2. This probe displayed desired properties such as high specificity, "Turn-On" fluorescence response with suitable sensitivity, appreciable water solubility and rapid response time (within 5 min). Moreover, as a biocompatible molecule, the probe has been successfully applied for imaging the hypoxic status of tumor cells (e. g. A549 cells) and especially used for real-time determination of nitroreductase produced by Escherichia coli. Therefore, we hope to apply this novel method in the biomedical research fields for the imaging of disease-relevant hypoxia and detection of pathogenic microorganisms.