文献类型：期刊文献

英文题名：Separation of polysaccharides from Lycium barbarum L. by high-speed countercurrent chromatography with aqueous two-phase system

作者：Cao, Yu[1,2,3];Huang, Xinyi[1,2];Di, Duolong[1,2,3];Wang, Han[1,2,4,5];Liu, Jianfei[1,2,3,4,5]

第一作者：Cao, Yu

通信作者：Wang, H[1];Liu, JF[1];Wang, H[2];Liu, JF[2]

机构：[1]Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China;[2]Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China;[3]Gansu Univ Tradit Chinese Med, Sch Pharm, Lanzhou 730000, Peoples R China;[4]Chinese Acad Sci, Key Lab Chem Northwestern Plant Resources, Lanzhou Chem Phys, 18 Tianshui Middle Rd, Lanzhou 730000, Peoples R China;[5]Chinese Acad Sci, Key Lab Nat Med Gansu Prov, Lanzhou Chem Phys, 18 Tianshui Middle Rd, Lanzhou 730000, Peoples R China

第一机构：Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China

通信机构：[1]corresponding author), Chinese Acad Sci, Key Lab Chem Northwestern Plant Resources, Lanzhou Chem Phys, 18 Tianshui Middle Rd, Lanzhou 730000, Peoples R China;[2]corresponding author), Chinese Acad Sci, Key Lab Nat Med Gansu Prov, Lanzhou Chem Phys, 18 Tianshui Middle Rd, Lanzhou 730000, Peoples R China.

年份：2024

卷号：256

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：;EI(收录号：20234915146633);Scopus(收录号：2-s2.0-85178384516);WOS:【SCI-EXPANDED(收录号:WOS:001129593700001)】；

基金：This work was financially supported by Youth Innovation Promotion Association CAS (2023442) , the Science and Technology Plan Project of Lanzhou City (2022-2-10) , and the Key Research and Development Program of the Ningxia Hui Autonomous Region (2021BEF02011, 2022BEG03169) . The authors also are grateful to anonymous reviewers for their comments and peer -review.

语种：英文

外文关键词：Lycium barbarum polysaccharides; High -speed countercurrent chromatography; Aqueous two-phase system; Decoloration; Separation; Hypoglycemic activity

摘要：The traditional method for isolation and purification of polysaccharides is time-consuming. It often involves toxic solvents that destroy the function and structure of the polysaccharides, thus limiting in-depth research on the essential active ingredient of Lycium barbarum L. Therefore, in this study, high-speed countercurrent chroma-tography (HSCCC) and aqueous two-phase system (ATPS) were combined for the separation of crude poly-saccharides of Lycium barbarum L. (LBPs). Under the optimized HSCCC conditions of PEG1000-K2HPO4-KH2PO4- H2O (12:10:10:68, w/w), 1.0 g of LBPs-ILs was successfully divided into three fractions (126.0 mg of LBPs-ILs-1, 109.9 mg of LBPs-ILs-2, and 65.4 mg of LBPs-ILs-3). Moreover, ATPS was confirmed as an efficient alternative method of pigment removal for LBPs purification, with significantly better decolorization (97.1 %) than the traditional H2O2 method (88.5 %). Then, the different partitioning behavior of LBPs-ILs in the two-phase system of HSCCC was preliminarily explored, which may be related to the difference in monosaccharide composition of polysaccharides. LBPs-ILs-1 exhibited better hypoglycemic activities than LBPs-ILs-2 and LBPs-ILs-3 in vitro. Therefore, HSCCC, combined with aqueous two-phase system, was an efficient separation and purification method with great potential for separating and purifying active polysaccharides in biological samples.