文献类型：期刊文献

中文题名：镰形棘豆总黄酮对特发性肺纤维化模型大鼠肺组织的改善作用研究

英文题名：Effect of total flavonoids from Oxytropis falcata Bunge on lung tissue in idiopathic pulmonary fibrosis model rats

作者：李欣泽[1,2,3];王彦君[1,2,3];李杨[1];梁乾坤[1];陈彦文[1];杨玲玲[1];明海霞[1,2]

第一作者：李欣泽

机构：[1]甘肃中医药大学基础医学院,甘肃兰州730000;[2]甘肃省高校重大疾病分子医学与中医药防治研究重点实验室,甘肃兰州730000;[3]甘肃中医药大学中西医结合研究所,甘肃兰州730000

第一机构：甘肃中医药大学基础医学院（敦煌医学研究所）

年份：2021

卷号：37

期号：15

起止页码：1999

中文期刊名：中国临床药理学杂志

外文期刊名：The Chinese Journal of Clinical Pharmacology

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

语种：中文

中文关键词：特发性肺纤维化;镰形棘豆总黄酮;α-平滑肌肌动蛋白;Ⅰ型胶原蛋白;Ⅲ型胶原蛋白

外文关键词：idiopathic pulmonary fibrosis;total flavonoids from Oxytropis falcata Bunge;alpha-smooth muscle actin;collagenⅠ;collagenⅢ

摘要：目的观察镰形棘豆总黄酮(FOFB)对特发性肺纤维化(IPF)模型大鼠α-平滑肌肌动蛋白(α-SMA)、Ⅰ型胶原蛋白(COLⅠ)和Ⅲ型胶原蛋白(COLⅢ)表达的影响。方法借助喉镜行气管插管和滴注博来霉素(5 mg·kg^(-1))建立IPF大鼠模型。按照体重将SD大鼠随机分为6组:空白组、模型组、低中高3个剂量实验组(100,200,400 mg·kg^(-1)镰形棘豆总黄酮)和阳性对照组(20 mg·kg^(-1)醋酸泼尼松),每组10只。造模后第15天,开始灌胃给药,1次/天,连续4周。以Masson染色法评价肺纤维化程度,以免疫组化法检测肺组织中α-SMA、COLⅠ和COLⅢ蛋白的表达(组织化学评分)。结果空白组、模型组、低中高3个剂量实验组和阳性对照组的肺纤维化评分分别为(0.00±0.00),(3.16±0.41),(2.82±0.41),(2.33±0.52),(0.50±0.54)和(0.33±0.52)分;这6组的α-SMA蛋白表达水平分别为(68.34±3.88),(127.94±1.66),(112.43±16.28),(95.88±3.71),(95.12±3.58)和(75.86±6.05)分;这6组的COLⅠ蛋白表达水平分别为(33.84±0.72),(52.48±5.44),(47.82±5.31),(43.06±1.66),(42.23±2.02)和(39.56±3.96)分;这6组的COLⅢ蛋白表达水平分别为(54.74±23.52),(124.00±2.90),(105.27±13.70),(97.34±9.13),(92.88±4.68)和(76.48±19.73)分。基因结果的趋势与蛋白一致。上述指标:模型组与空白组相比,差异均有统计学意义(均P<0.05);中、高2个剂量实验组和阳性对照组与模型组比较,差异均有统计学意义(均P<0.05)。结论FOFB可能通过下调α-SMA、COLⅠ、COLⅢ的表达而延缓博来霉素诱导的IPF大鼠的纤维化进展。
Objective To observe the effect and mechanism of total flavonoids from Oxytropis falcata Bunge(FOFB)onα-smooth muscle actin(α-SMA),collagenⅠ(COLⅠ)and collagenⅢ(COLⅢ)in idiopathic pulmonary fibrosis(IPF)model rats.Methods IPF model rats were established by endotracheal intubation with laryngoscope and bleomycin(5 mg·kg^(-1)).SD rats were randomly divided into 6 groups according to weight:blank group model group,experimental-L,experimental-M,experimental-H groups(100,200 and 400 mg·kg^(-1) FOFB),positive control group(20 mg·kg^(-1) prednisone acetate),each group included 10 animals.On the 15 th day after modeling,the rats were given intragastric administration once a day for 4 weeks.The degree of pulmonary fibrosis was evaluated by Masson staining.The expression(histochemistry score)ofα-SMA,COLⅠand COLⅢproteins in lung tissue were determined by immunohistochemistry.Results The pulmonary fibrosis score in blank group,model group,experimental-L,experimental-M,experimental-H groups and positive control group were(0.00±0.00),(3.16±0.41),(2.82±0.41),(2.33±0.52),(0.50±0.54),(0.33±0.52)point,respectively;the protein expression level ofα-SMA in the six groups were(68.34±3.88),(127.94±1.66),(112.43±16.28),(95.88±3.71),(95.12±3.58)and(75.86±6.05)point,respectively;the protein expression level of COLⅠin the six groups were(33.84±0.72),(52.48±5.44),(47.82±5.31),(43.06±1.66),(42.23±2.02)and(39.56±3.96)point,respectively;the protein expression level of COLⅢin the six groups were(54.74±23.52),(124.00±2.90),(105.27±13.70),(97.34±9.13),(92.88±4.68)and(76.48±19.73)point,respectively.The trend of gene results was consistent with that of protein.Significant difference of the factors were found between blank group and model group(all P<0.05);There had statistical significance comparison between experimental-M group,experimental-H group,positive control group and model group(all P<0.05).Conclusion FOFB may delay bleomycin-induced fibrosis in IPF rats by down-regulating the expression ofα-SMA,COLⅠand COLⅢ.