文献类型：期刊文献

英文题名：Integrated Approach for Separation of 2-O-β-D-glucopyranosyl-L-ascorbic Acid From Lycium barbarum L. Fruit Using High-Speed Countercurrent Chromatography Coupled With Aqueous Two-Phase System

作者：Chen, Yuliang[1];Zhang, Huali[1];Ma, Ling[2];Wang, Xiaofang[3];Yang, Jinjin[1];Wang, Ningli[4,5];Pei, Dong[1,4,5,6];Di, Duolong[1,4,5]

第一作者：陈亚兰;陈月玲

通信作者：Pei, D[1];Di, DL[1];Wang, NL[2];Pei, D[2];Di, DL[2];Wang, NL[3];Pei, D[3];Di, DL[3];Pei, D[4]

机构：[1]Gansu Univ Chinese Med, Coll Pharm, Lanzhou, Peoples R China;[2]Ningxia Inst Drug Control, Yinchuan, Peoples R China;[3]Gansu Pharmaceut Grp Sci & Technol Res Inst, Lanzhou, Peoples R China;[4]Chinese Acad Sci, Lanzhou Inst Chem Phys, CAS Key Lab Chem Northwestern Plant Resources, Lanzhou, Peoples R China;[5]Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou, Peoples R China;[6]Qingdao Ctr Resource Chem & New Mat, Qingdao, Peoples R China

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Coll Pharm, Lanzhou, Peoples R China;[2]corresponding author), Chinese Acad Sci, Lanzhou Inst Chem Phys, CAS Key Lab Chem Northwestern Plant Resources, Lanzhou, Peoples R China;[3]corresponding author), Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou, Peoples R China;[4]corresponding author), Qingdao Ctr Resource Chem & New Mat, Qingdao, Peoples R China.|[1073501e14fb35863569f]甘肃中医药大学药学院（西北中藏药协同创新中心办公室）;[10735]甘肃中医药大学;

年份：2026

卷号：49

期号：1

外文期刊名：JOURNAL OF SEPARATION SCIENCE

收录：;EI(收录号：20260620015913);Scopus(收录号：2-s2.0-105028929332);WOS:【SCI-EXPANDED(收录号:WOS:001703768100001)】；

基金：We sincerely thank the financial support from the Key R&D Program of Ningxia Hui Autonomous Region (2024BBF02008), Gansu Science Fund for Basic Creative Research Groups (25JRRA469), State-owned capital budget supports scientific research projects of provincial enterprises (2024GZ021), Science Foundation of Shandong Province (ZR2024MB018), Key R&D Program of Gansu Province (25YFFA033), and the Qingdao Municipal Bureau of Science and Technology (No. 24-1-4-xxgg-17-nsh).

语种：英文

外文关键词：2-O-beta-D-glucopyranosyl-L-ascorbic acid; aqueous two-phase system; entropy weight method; high-speed counter current chromatography; overlapping injection mode; response surface methodology

摘要：2-O-beta-D-glucopyranosyl-L-ascorbic acid (AA-2 beta G), a natural ascorbic acid derivative discovered in Lycium barbarum L., has been demonstrated to exhibit multiple biological activities. This study developed an integrated approach combining high-speed counter-current chromatography (HSCCC) and an aqueous two-phase system (ATPS) for the preparative isolation of AA-2 beta G from L. barbarum fruit. The solution system of ammonium sulfate/ethanol/water/acetonitrile (19.5:28.9:53.3:2.0, w/w) was established through systematic screening processes. To address the multi-parameter optimization challenges in HSCCC, this study employed the entropy weight method to calculate the information entropy and weight coefficients of five critical parameters, thereby establishing a comprehensive evaluation methodology for HSCCC separation efficiency. Subsequently, the response surface methodology was utilized to optimize HSCCC operational conditions. Under the optimized conditions, 2.66 mg of AA-2 beta G with a purity of 91.24% was successfully isolated from a 140 mg extract. Building on the optimized HSCCC conditions, an overlapping injection mode was implemented based on the conventional single-injection mode. Through cumulative purification of 700 mg extract, 10.65 mg of AA-2 beta G with an 84.53% purity was successfully obtained. Compared with the single-injection mode, this mode reduced separation time by 55.68% while decreasing solvent consumption by 36.22%. Finally, the antioxidant activity of AA-2 beta G was assessed through in vitro antioxidant activity experiments. These results demonstrate the synergistic potential of the HSCCC-ATPS integration as an efficient strategy for bioactive compound purification. The established protocol provides a robust method for AA-2 beta G preparation from Lycium barbarum L. fruit. Furthermore, it demonstrates the broader applicability of this hybrid technique in isolating bioactive constituents from complex biological matrices.