文献类型：期刊文献

中文题名：荣骨定痛膏的镇痛抗炎作用及其机制

英文题名：The antalgic and antiphlogistic function and mechanism of RGDT plaster

作者：刘晓霞[1];王志旺[1];陈小平[2];刘彩民[2];妥海燕[1]

第一作者：刘晓霞

机构：[1]甘肃中医学院,兰州730000;[2]甘肃省靖远县中医院,靖远730600

第一机构：甘肃中医药大学

年份：2015

卷号：31

期号：5

起止页码：462

中文期刊名：中国应用生理学杂志

外文期刊名：Chinese Journal of Applied Physiology

收录：CSTPCD;;Scopus;CSCD:【CSCD2015_2016】；PubMed;

基金：甘肃省中医药管理局科研课题基金项目(GZK-2013-38)

语种：中文

中文关键词：荣骨定痛膏;镇痛;抗炎;白三烯B4;小鼠

外文关键词：ronggudingtong plaster （RGDT plaster） ; analgesia; anti-inflammatory; LTB4 ; mice

摘要：目的:研究荣骨定痛膏镇痛、抗炎作用及其机制。方法:采用热板法、醋酸扭体法来观测荣骨定痛膏的镇痛作用,采用耳廓肿胀法、足肿胀法来考察荣骨定痛膏的抗炎作用,测定渗出液中总蛋白与白三烯B4(LTB4)来研究荣骨定痛膏的抗炎机制。在给药的基础上观测痛阈值、肿胀度等指标;复制气囊滑膜炎,取小鼠66只,随机分为正常对照组、模型对照组、阳性对照组(扶他林0.8 mg/d)、荣骨定痛膏低、中、高剂量组(荣骨定痛膏30 mg/d、60 mg/d、120 mg/d)进行实验。结果:30 mg/d、60 mg/d、120 mg/d的荣骨定痛膏可明显提高小鼠热板痛阈延长百分率、延长扭体潜伏期并减少15 min内的扭体次数,缓解小鼠耳廓肿胀度与足肿胀度(P<0.05,P<0.01),显示出一定的抗炎、镇痛作用。对气囊滑膜炎模型动物,30 mg/d、60 mg/d、120 mg/d荣骨定痛膏可降低渗出物中总蛋白及LTB4的含量(P<0.05,P<0.01)。结论:荣骨定痛膏具有一定的镇痛抗炎作用,抑制LTB4的合成是其抗炎机制之一。
Objective： To study the antalgic and antiphlogistic functions and mechanism of ronggudingtong （RGDT） plaster（traditional Chinese medicine）. Methods： The painful models were established with hot plate test or acetic acid writhing and the inflarmlmatory models were established with daubing dimethylbenzene on auricle or injecting formaldehyde in toe or synovial envelope to study the antalgic and antiphlogistic functions of RGDT Plaster. The total protein and leukotriene 134 （LTB4） in inflammatory exudate were detected to investigate the antalgic and antiphlogistic mechanism of RGDT plaster. The mice were randomly divided into different groups（ n = 11 ）, on the basis of drug using, the in- dexes of pain threshold, swelling degree were observed. Sixty-six mice were used to establish gasbag synovitis model and randomly divided into normal control group, model control group, positive control group（Voltaren gel 0.8 mg/d）and low/medium/high dosage RGDT plaster treating groups（30 rag/d,60 mg/d, 120 nag/d）. Results： 30 mg/d,60 mg/d,120 mg/d RGDT plaster could upgrade the pain thresholds, remit au- ricular and foot swelling（ P 〈 0.05, P 〈 0.01 ）, and degrade total protein and LTB4 in inflammatory exudates（ P 〈 0.05, P 〈 0.01 ）. Con- elusion： RGDT plaster has some antalgic and antiphlogistic functions, and one of the mechanisms is depressing synthesis of LTB4.