文献类型：期刊文献

英文题名：Efficiency of ISSR marker in assessing the genetic diversity of wild and cultivated Hedysarum polybotrys Hand. Mazz

作者：Qiang, Zhengze[1];Wang, Yan[1];Li, Shuo[1];Wang, Mingwei[1];Luo, Xudong[1];Li, Xu[1];Feng, Xiaoli[1];Li, Chengyi[1]

第一作者：Qiang, Zhengze

通信作者：Li, CY[1]

机构：[1]Gansu Univ Tradit Chinese Med, Dept Pharm, Lab Identificat & Qual Evaluat Chinese Med Herbs, Lanzhou, Gansu, Peoples R China

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

通信机构：[1]corresponding author), Gansu Univ Tradit Chinese Med, Dept Pharm, Lab Identificat & Qual Evaluat Chinese Med Herbs, Lanzhou, Gansu, Peoples R China.|[1073501e14fb35863569f]甘肃中医药大学药学院（西北中藏药协同创新中心办公室）;[10735]甘肃中医药大学;

年份：2018

卷号：71

期号：2

起止页码：174

外文期刊名：CARYOLOGIA

收录：;Scopus(收录号：2-s2.0-85046442837);WOS:【SCI-EXPANDED(收录号:WOS:000433932200013)】；

基金：This paper was supported by the National Natural Science Foundation of China [81360621].

语种：英文

外文关键词：Hedysarum polybotrys Hand. Mazz; ISSR; cultivated; wild; genetic diversity

摘要：Genetic variation of cultivated and wild populations plays an important role in maintaining the population gene polymorphism and developing future management strategies. Hedysarum polybotrys is an important economic plant in Gansu Province, China. This paper was the first to investigate and analyse the genetic diversity between wild and cultivated H. polybotrys. The results of the ISSR-PCR revealed that 479 (98.97%) of the 484 ISSR loci tested by 17 primers were polymorphic. The change of PPL was 92.98-93.80%. The species level of the alleles, the effective number of alleles, the Nei's gene diversity and the Shannon's information index were 1.9897, 1.6006, 0.3502 and 0.5228, respectively. The high-low sequence of the genetic diversity of the wild population was greater than that of the cultivated population. The genetic distance and genetic identity between the cultivated and wild populations were 0.0488 and 0.9524. AMOVA analysis indicated that genetic variation mainly occurred among populations (8.09%, 6.47%, respectively). UPGMA analysis revealed that the 52 samples were clustered into two branches, I and II. Although most of the alleles from the wild populations were maintained in the gene pool of the cultivated populations, a small amount of the alleles remained in the wild population. PCoA and population structure analysis confirmed the partitioning results of the UPGMA clustering. The H. polybotrys populations had a high level of genetic diversity, and the genetic differentiation of two populations was low. Results provided a theoretical basis for the protection and utilisation of H. polybotrys resources.