文献类型：期刊文献

中文题名：短小芽孢杆菌DST27发酵培养基及发酵条件的优化

英文题名：Optimization of fermentation medium and conditions for Bacillus pumilus DST27

作者：李铮[1];王艳[1,2,3];晋玲[1,2];王伟[1];付瑶[1];栾涵钰[1];吕娇娇[1]

第一作者：李铮

机构：[1]甘肃中医药大学药学院,兰州730000;[2]西北中藏药省部共建协同创新中心,兰州730000;[3]甘肃省中医药研究中心,兰州73000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2024

卷号：63

期号：7

起止页码：500

中文期刊名：农药

外文期刊名：Agrochemicals

收录：CSTPCD;;北大核心:【北大核心2023】；CSCD:【CSCD_E2023_2024】；

基金：西北中藏药省部共建协同创新中心开放基金项目(Xbzzy-2022-02);中药保障与创新能力提升项目(甘中医药综函[2024]14号);甘肃省科技计划项目(23ZDFA013-1);甘肃省2023年度优秀研究生“创新之星”(2023CXZX-763);甘肃省中医药研究中心开放课题(zyzx-2023-20)。

语种：中文

中文关键词：短小芽孢杆菌DST27;培养基优化;发酵条件优化;Plackett-Burman设计;响应面分析法

外文关键词：Bacillus pumilus DST27;medium optimization;optimization of fermentation conditions;Plackett-Burman design;response surfaceanalysis

摘要：[目的]通过优化生防菌株短小芽孢杆菌DST27发酵培养基及发酵培养条件,为后期该菌株的开发和应用提供基础。[方法]通过单因素筛选、Plackett-Burman试验和响应面分析法,以菌体生长量和对尖孢镰刀菌的抑菌活性为指标,优化DST27发酵培养基及发酵培养条件。[结果]最佳发酵培养基为1.35%葡萄糖、1%酵母粉和0.5%CaCl2;最佳发酵条件为装液量20%、接种量5.40%、初始pH 7.28、温度28℃和转速180 r/min,在此培养条件下DST27的OD600为2.256,抑菌率为78.65%,与模型预测值81.42%相近,较优化前提高了22.06%。[结论]该试验优化了DST27发酵培养基及发酵条件,为今后该菌剂生产工艺及开展防治提供理论依据。
[Aims]This study aims to establish a foundation for the later development and use of the biocontrol strain Bacillus pumilus DST27 by optimizing the fermentation medium and circumstances.[Methods]Based on single factor test,Plackett-Burman test and response surface analysis,the fermentation medium and conditions of DST27 were optimized with the bacterial biomass and the antifungal activity against Fusarium oxysporum as indicators.[Results]The optimal medium for liquid fermentation of strain DST27 was 1.35%glucose,1%yeast powder,and 0.5%CaCl.The optimal fermentation conditions were as follows:liquid-loading rate 20%,inoculation amount 3%,pH 7.28,temperature 28℃,time 60 h,and rotating speed 180 r/min.The ODoo of DST27 was 2.256 under these cultivation circumstances,and the antifungal rate was 78.65%,which was similar to the predicted value of 81.42%in the model,and had increased by 22.06%compared to that before optimization.[Conclusions]This experiment optimized the fermentation medium and conditions of DST27,providing a theoretical basis for the production process of this bacterial agent and prevention with it in the future.