文献类型：期刊文献

英文题名：An α-hemoglobin-derived peptide (m)VD-hemopressin (α) promotes NREM sleep via the CB₁ cannabinoid receptor

作者：Xie, Jun-Fan[1];Wang, Lin-Xin[1,2,3,4];Ren, Wen-Ting[1];Wang, Can[1,5];Gao, Jin-Xian[1,6];Chen, Hai-Lin[1];Zhao, Xue-Qi[1];Ren, Yan-Li[1];Xie, Yu-Ping[6];Shao, Yu-Feng[1];Hou, Yi-Ping[1]

第一作者：Xie, Jun-Fan

通信作者：Hou, YP[1]

机构：[1]Lanzhou Univ, Sch Basic Med Sci, Dept Neurosci, Key Lab Preclin Study New Drugs Gansu Prov, Lanzhou, Peoples R China;[2]Gansu Univ Chinese Med, Sch Basic Med Sci, Dept Anat, Lanzhou, Peoples R China;[3]Gansu Univ Chinese Med, Sch Basic Med Sci, Dept Histol, Lanzhou, Peoples R China;[4]Gansu Univ Chinese Med, Sch Basic Med Sci, Dept Embryol, Lanzhou, Peoples R China;[5]Weifang Med Univ, Sch Basic Med Sci, Dept Anat, Weifang, Peoples R China;[6]Gansu Prov Hosp, Sleep Med Ctr, Lanzhou, Peoples R China

第一机构：Lanzhou Univ, Sch Basic Med Sci, Dept Neurosci, Key Lab Preclin Study New Drugs Gansu Prov, Lanzhou, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Sch Basic Med Sci, Dept Neurosci, Key Lab Preclin Study New Drugs Gansu Prov, Lanzhou, Peoples R China.

年份：2023

卷号：14

外文期刊名：FRONTIERS IN PHARMACOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001030067600001)】；

基金：This study was supported by grants from the National Natural Science Foundation of China (82001396, 81771426, 81471347, 31872770, 31500853, and 82160267), the program of the Gansu Provincial Science and Technology Department (20JR5RA228), the Gansu Provincial Science and Technology Program Projects (21YF5FA020), the China Scholarship Council (201906185012), the Talent-Introducing Project of State Administration of Foreign Experts Affairs of China (G2022175003L and X2017008), and the Fundamental Research Funds for the Central University of China (lzujbky-2021-39, lzujbky-2019-cd03, and lzujbky-2018-25).

语种：英文

外文关键词：mouse VD-hemopressin (& alpha;); endocannabinoid; cannabinoid receptors; cannabinoid receptors antagonist; c-Fos; sleep-wake states

摘要：Hemopressin and related peptides have shown to function as the endogenous ligands or the regulator of cannabinoid receptors. The previous studies demonstrated that the endocannabinoid system played important roles in modulating several physiological functions such as sleep, olfaction, emotion, learning and memory, and reward behaviors. Mouse VD-hemopressin (a) [(m)VD-HPa], an 11-residue peptide derived from the a1 chain of hemoglobin, was recently presumed as a selective agonist of the CB1 receptor. The present study was undertaken to investigate the effects of (m)VD-HPa on the sleep-wake cycle and power spectrum of cortical EEG in freely moving rats and the potential neurons in the brain activated by (m)VD-HPa. The results showed that 20.1 nmol of (m)VD-HPa i.c.v. administration increased non-rapid eye movement (NREM) sleep in the first 2 h section accompanied by an increase in EEG delta (0.5-4 Hz) activity. The (m)VD-HPa-induced NREM sleep enhancement was due to extended episode duration instead of the episode number. In addition, the effect of (m)VD-HPa (20.1 nmol) on sleep-wake states was significantly attenuated by an antagonist of the CB1 receptor, AM251 (20 nmol, i.c.v.) but not by the CB2 receptor antagonist, AM630 (20 nmol, i.c.v.). In comparison with vehicle, (m)VD-HPa increased Fos-immunoreactive (-ir) neurons in the ventrolateral preoptic nucleus (VLPO), but reduced Fos-ir neurons in the lateral hypothalamus (LH), tuberomammillary nucleus (TMN), and locus coeruleus (LC). These findings suggest that (m)VD-HPa promotes NREM sleep via the CB1 cannabinoid receptor to probably activate VLPO GABAergic neurons, but inactivates the LH orexinergic, LC noradrenergic, and TMN histaminergic neurons.