文献类型：期刊文献

中文题名：当归有效成分对冷应激小鼠抗氧化功能影响

英文题名：Effects of active fraction of Angelica sinensis on antioxidant function in cool stress mice

作者：骆亚莉[1];李应东[2];刘永琦[1];夏鹏飞[3];颜春鲁[1];孙丽姣[4];蔡路路[4]

第一作者：骆亚莉

机构：[1]甘肃中医学院系统生物学与中医药转化研究所,甘肃兰州730000;[2]甘肃中医学院附属医院;[3]甘肃中医学院科研中心;[4]甘肃中医学院预防医学2011级本科

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2014

卷号：30

期号：5

起止页码：607

中文期刊名：中国公共卫生

外文期刊名：Chinese Journal of Public Health

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD_E2013_2014】；

基金：国家“十二五"科技支撑计划(2011BAI05B02)

语种：中文

中文关键词：冷应激;当归有效成分;抗氧化

外文关键词：cool stress; active fraction of Angelica sinensis; antioxidant

摘要：目的观察当归有效成分对冷应激小鼠抗氧化、红细胞粘附功能的影响。方法采用水提醇沉法提取当归3种有效成分(A、B、C);72只SPF小鼠随机分为对照、模型、当归有效成分A、B、C、红景天共6组;灌胃给药,连续14 d;从第10 d开始,灌胃后0.5 h小鼠置于(8±2)℃水中游泳3 min,连续5 d,建立冷应激模型;小鼠断头处死,取血备用,记录动物断头后张口呼吸时间、心搏时间,计数胸腺、脾脏指数,检测脾脏、胸腺谷胱甘肽过氧化物酶(GSH-Px)、血清超氧化物歧化酶(SOD)水平、丙二醛(MDA)含量、红细胞粘附能力。结果与对照组比较,模型组小鼠断头后张口呼吸时间、心搏时间[分别为(20.75±3.11)、(51.63±4.10)s]缩短,胸腺、脾脏指数[分别为(2.19±0.47)、(2.62±0.61)mg/g]下降,脾脏、胸腺GSH-Px含量[分别为(19.16±2.15)、(1.65±0.58)U/mgpro]、血清SOD含量[(173.53±27.39)U/mL]均降低,MDA含量[(12.02±0.62)nmol/mL]升高(P<0.05),红细胞免疫复合物花环率(RBC-IC)、红细胞C3b受体花环率(RBC-C3bR)下降;与模型组比较,当归各组小鼠断头后心搏时间均延长,胸腺、脾脏指数升高(P<0.05);当归B、C组小鼠胸腺组织GSH-Px含量[分别为(5.32±2.47)、(3.76±1.58)U/mgpro]和RBC-IC花环率均升高(P<0.05),当归B组小鼠血清SOD水平[(220.03±36.33)U/mL]升高、血清MDA含量[(7.95±1.55)nmol/mL]降低、脾脏组织GSH-Px含量[(49.49±1.77)U/mgpro]和RBC-C3bR花环率升高(P<0.05)。结论当归有效成分B、C能增强冷应激小鼠抗氧化能力,提高红细胞粘附功能。
Objective To determine the effects of active fraction of Angelica sinensis on antioxidant and red cell im- mune adherence function in cool stress mice. Methods The water extraction and alcohol precipitation method were adopted to obtain 3 active fractions of Angelica sinensis. Seventy-two healthy SPF mice were randomly divided into con- trol group（ M）, model group （K） ,Angelica sinensis active fraction A treatment group （A） ,Angelica sinensis active frac- tion B treatment group（ B ） ,Angelica sinensis active fraction C treatment group（C） ,Rhodiola rosea treatment group（T）. The mice were administered corresponding treatment by gavage for 14 days. Control mice were given normal saline in same volume. On the 10th day of the treatment,3 minutes forced cold water（ 8 ± 2 ~C ） swimming 0. 5 hour after gavage treatment was used as a stressor to all mice excepted control mice once a day for 5 days. On the 14th day of the treat- ment, all mice were decapitated and duration of gasping and heart beat after decapitation were investigated. And the blood samples were collected to determine erythrocytic immune function and the content of superoxide dismutase （SOD） and malondialdehyde（MDA） in blood serum. Immune organ weight of the mice was observed and the level of glutathione per- oxidase （GSH-Px） in immune organ tissue was determined. Results Compared with the control group, duration of gasp- ing （20. 75 ± 3.11 s ） and heart beat （ 51.63 ± 4. 10 s ） after decapitation, thymus （ 2. 19 ± 0. 47 mg/g ） and spleen index （2. 62 ± 0. 61 mg/g）decreased significantly in the model group（ P 〈 0. 05 ）. The content of GSH-Px in thymus and spleen （19. 16± 2. 15,1.65 e0. 58 U/mgpro）, the level of SOD （173.53±27.39 U/mL）, and red blood cell C 3b receptor （ RBC-C3 bR） rosette rate, red blood cell immune complex （RBC-IC） rosette rate decreased significantly, but the level of MDA in serum（ 12. 02± 0. 62 nmol/mL）significantly increased in the model group（P 〈0. 05 ）. Experiment tests showed that the duration of heart beat after decapitation prolonged, the thymus and spleen index increased in the mice of group A, B, and C compared with those of the model group （P 〈 0. 05 for all）. The content of GSH-Px in thymus in the mice of group B （5.32 ± 2.47 U/mgpro）and group C （3.76 ± 1.58U/mgpro）, and RBC-IC rosette rate in both groups increased compared with those of the model group（ P 〈 0. 05 for all）. The level of serum SOD（ 220. 03 ± 36. 33 U/mL）, the con- tent of GSH-Px in spleen （49.49 ± 1.77 U/mgpro）, and RBC-C3bR rosette rate were obviously increased but MDA （7. 95 ± 1.55 nmol/mL）decreased in group B compared with those of the model group（ P 〈 0. 05 for all）. Conclusion Active fraction B and C of Angelica sinensis could enhance the antioxidant ability and strengthen the erythrocytic immune function in cool stress mice.