文献类型：期刊文献

英文题名：Pharmacodynamics, metabolomics and pathological studies on mechanisms of traditional benefits of Angelica sinensis in blood circulation

作者：Yang, Xiujuan[1];Hai, Yunxiang[2];Yang, Zhijun[2];Wang, Pengqian[1];Li, Jiameng[1];Hao, Danli[1];Zhang, Miao[1];Deng, Yi[2];Sui, Feng[1]

第一作者：Yang, Xiujuan

通信作者：Sui, F[1]

机构：[1]China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing 100700, Peoples R China;[2]Gansu Univ Tradit Chinese Med, Lanzhou 730000, Peoples R China

第一机构：China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing 100700, Peoples R China

通信机构：[1]corresponding author), China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing 100700, Peoples R China.

年份：2023

卷号：22

期号：1

起止页码：122

外文期刊名：INDIAN JOURNAL OF TRADITIONAL KNOWLEDGE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001022147600015)】；

基金：Acknowledgements This study was supported by the National Natural Science Foundation of China (No. 81873024, 82173998) , Postdoctoral Science Foundation of China (No. 2020M670600) , Natural Science Foundation of Gansu Province (No. 20JR10RA331) , Gansu Provincial Department of Education Project (No. 2021B-157) . The funding agencies did not have any role in study design, data collection and analyses, decision on publication, or preparation of the manuscript.

语种：英文

外文关键词：Activating blood; Angelica sinensis; Metabolomics and pathological studies; Pharmacodynamics; Traditional benefits

摘要：Angelica sinensis is a rich source of medically important active molecules that need in-depth understanding on its action mechanisms. Therefore, through pharmacodynamics, metabolomics, and network pharmacology, the traditional benefits of A. sinensis in blood circulation was studied using 24 randomly selected Sprague-Dawley (SD) rats. Measurement of the blood rheological parameters for whole blood viscosity (WBV) and plasma viscosity (PV), and inspection of the heart and lung tissues pathological changes were undertaken using molecular and bioinformatic techniques. Multivariate statistical analysis and establishment of the model of the relationship between metabolite expression and sample categories to test the prediction of sample categories were performed. Screening was undertaken to find the potential metabolites for A. sinensis to treat blood stasis syndrome and find related metabolic pathways. Active ingredients of A. sinensis and targets and building of an "effect component-target" network was undertaken, A. sinensis was confirmed to improve blood stasis syndrome in rats and improve heart and lung pathology to varying degrees. Compared with the blood stasis model group, A. sinensis significantly reduced WBV and PV in hemorheology (p<0.05, p<0.01) and regulated blood stasis-induced changes in 22 metabolites including alpha-D-glucose, L-isoleucine, creatine and acetylcarnitine, which are involved in the metabolism of linoleic acid, linolenic acid, phenylalanine, ascorbic acid and uronic acid. Using the network pharmacology to build a "component-target-pathway" network of A. sinensis, 62 active ingredients, 169 active proteins and 18 metabolic pathways were obtained, among which linoleic acid metabolism, ascorbic acid and uronic acid metabolism were consistent with the metabolic pathways obtained by metabolomics.