文献类型：期刊文献

中文题名：赤芍与藜芦配伍对小鼠肝脏及肾脏损伤的影响

英文题名：Effect on Liver and Kidney of Mice by Traditional Chinese Medicine Compatibility of Paeoniae Radix Rubra and Veratrum Nigrum

作者：姚凝[1]

第一作者：姚凝

机构：[1]甘肃中医学院临床医学院,兰州甘肃730000

第一机构：甘肃中医药大学临床医学院

年份：2014

卷号：21

期号：5

起止页码：46

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2013_2014】；

基金：甘肃省自然科学基金(0710RJZA010)

语种：中文

中文关键词：赤芍;藜芦;十八反;肝;肾;氧化-抗氧化平衡;小鼠

外文关键词：Paeoniae radix rubra;Veratrum nigrum;eighteen incompatible medicaments;liver;kidney;oxidation-antioxidation balance;mice

摘要：目的观察赤芍与藜芦不同比例配伍对小鼠肝脏及肾脏损伤的影响,并探讨其机制。方法赤芍与藜芦按照单药使用及4∶1、2∶1、1∶1、1∶2、1∶4不同比例配伍水煎对小鼠灌胃22 d,取外周血检测血清丙氨酸氨基转移酶、天冬氨酸氨基转移酶(AST)、乳酸脱氢酶活性及血清肌酐、尿素氮含量,取肝脏及肾脏组织检测组织谷胱甘肽过氧化物酶(GSH-Px)活性及丙二醛(MDA)含量。结果不同比例赤芍、藜芦配伍使用对小鼠肝脏损伤作用不明显,但导致血清AST活性增高;不同比例赤芍、藜芦配伍使用对小鼠肾脏损伤作用明显,其中赤芍-藜芦2∶1比例配伍损伤最严重,同时肾脏组织GSH-Px活性降低伴MDA含量升高。结论赤芍、藜芦不同比例配伍使用可引起小鼠肾脏损伤,其原因与肾脏组织氧化-抗氧化平衡紊乱相关。
Objective To investigate the damage of mice liver and kidney with the compatibility application of Paeoniae radix rubra and Veratrum nigrum, and explore its mechanism. Methods We examined the alanine aminotransferase, aspartate aminotransferase （AST）, lactic dehydrogenase, creatinine and urea nitrogen from serum, and glutathion peroxidase （GSH-Px） and malondialdehyde （MDA） from liver and kidney tissue after intragastric administration of different ratio （4∶1, 2∶1, 1∶1, 1∶2, 1∶4） of Paeoniae radix rubra and Veratrum nigrum in mice for 22 d. Results Paeoniae radix rubra used with Veratrum nigrum did not induce the obvious damage to liver with AST increase, but induced the obvious damage to kidney. At the same time, the depressed GSH-Px activity and the increased MDA content were observed in kidney tissue. When Paeoniae radix rubra and Veratrum nigrum is on a ratio of 2∶1, the kidney injury was the most obvious. Conclusion Paeoniae radix rubra used with Veratrum nigrum in varying proportions could induce the kidney injury in mice which is related to the oxidation-antioxidation balance disturbance.