文献类型：期刊文献

中文题名：光合细菌沼泽红假单胞菌DG43促进当归增产提质的理化机理

英文题名：Physicochemical mechanisms underpinning the improving effects of Rhodopseudomonas palustris DG43 on the yield and quality of Angelica sinensis

作者：周绪坤[1];张一群[1];杨涛[1,2];王琼[4];林晓丽[4];赵文龙[1,3]

第一作者：周绪坤

机构：[1]甘肃中医药大学药学院,甘肃兰州730000;[2]甘肃省科学院生物研究所甘肃省微生物资源开发利用重点实验室,甘肃兰州730000;[3]陇药产业创新研究院,甘肃兰州730000;[4]岷县园艺指导站,甘肃定西743000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2026

卷号：53

期号：5

起止页码：2460

中文期刊名：微生物学通报

外文期刊名：Microbiology China

收录：;北大核心:【北大核心2023】；

基金：甘肃省科技重大专项(23ZDFA013-1);中央引导地方科技发展资金项目(24ZYQA041);甘肃省科学院产业化项目(2024YLCY-01);平凉市重点研发项目(PL-STK-2024A-025);科技特派员(团)专项(25CXNJ007)。

语种：中文

中文关键词：沼泽红假单胞菌;当归;提质;增产;光合细菌

外文关键词：Rhodopseudomonas palustris;Angelica sinensis;quality enhancement;yield increasing;photosynthetic bacteria

摘要：【背景】当归作为我国传统名贵中药材,规模化种植中连作障碍突出,表现为土壤恶化、酚酸类自毒物质积累及土传病害高发,严重制约其产量、品质及产业可持续发展。沼泽红假单胞菌作为有益不放氧光合细菌,已在多种作物中展现促生、抗病及品质提升效应,但在当归中的应用及互作机制尚未明确。【目的】研究沼泽红假单胞菌(Rhodopseudomonas palustris)对当归生理生化、土壤养分活化利用、产量、品质的影响,阐明其与当归互作的机理,为不放氧光合细菌在当归绿色种植中的应用奠定理论基础。【方法】通过液相色谱-质谱联用技术(liquid chromatography-tandem mass spectrometry,LC-MS/MS)分析叶片激素代谢;采用试剂盒所述分光光度法检测植物碳氮代谢、抗氧化、光合相关理化指标,土壤养分及代谢循环、总酚酸含量等相关生理生化指标;常规方法统计当归生长指标、病害情况;高效液相色谱法(high performance liquid chromatography,HPLC)测定当归根中有效成分含量;相关性分析揭示与产量、品质相关的指标。【结果】叶面喷施沼泽红假单胞菌显著提高了当归叶片玉米素核苷(trans-zeatin riboside,tZR)、双氢玉米核苷-O-糖苷(dihydrozeatin-O-glucoside riboside,DHZROG)、反式-玉米素-O-葡萄糖苷(transzeatin-O-glucoside,tZOG)、双氢玉米素-7-糖苷(dihydrozeatin-7-glucoside,DHZ7G)、反式玉米素(trans-zeatin,tZ)、1-氨基环丙烷羧酸(1-aminocyclopropanecarboxylic acid,ACC)含量(P<0.05)。相较于对照组,沼泽红假单胞菌(Rhodopseudomonas palustris)(ZZH)处理组叶绿素含量提升12%,超氧阴离子清除率提高20%,2,2′-联氮-双-(3-乙基苯并噻唑啉-6-磺酸)[2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)radical scavenging capacity,ABTS]自由基清除力提高6%,总酚含量提高16%,土壤总酚酸含量降低25%(P<0.05);乙醇酸氧化酶(glycolate oxidase,GO)、谷氨酰胺合成酶(glutamine synthetase,GS)等5种酶活性显著增加(P<0.05)。当归身长、主根长和百株重分别增加27%、21%、10%;根腐病发病率降低24%(P<0.05);阿魏酸、阿魏酸松柏酯含量分别提高11%、36%(P<0.05)。总酚酸与根腐病病害率呈正相关、与百株重呈负相关;而ACC与根腐病病害率呈负相关、与百株重呈正相关;阿魏酸及阿魏酸松柏酯与ACC、玉米素核苷呈极显著正相关。【结论】沼泽红假单胞菌主要通过调控内源激素,尤其是ACC、玉米素类,降解酚酸类自毒物质,提高活性氧清除能力,增加当归产量、提高品质。
[Background]Angelica sinensis,a highly regarded traditional Chinese medicine,faces significant challenges associated with continuous cropping in large-scale cultivation.These challenges are characterised by soil degradation,the accumulation of phenolic acid self-toxicity,and a heightened incidence of soil-borne diseases,all of which severely impede its yield,quality,and the sustainable development of the industry.Rhodopseudomonas palustris,a beneficial anoxygenic photosynthetic bacteria,has demonstrated growth promotion,disease resistance,and quality enhancement in various crops;however,the mechanisms underlying its application and interaction with A.sinensis remain unclear.[Objective]This study investigates the effects of Rhodopseudomonas palustris on the physiological and biochemical characteristics,soil nutrients,yield,and quality of A.sinensis and elucidates the mechanisms underlying their mutual interactions,aiming to lay a theoretical foundation for the application of anoxygenic photosynthetic bacteria in the green cultivation of A.sinensis.[Methods]The leaf hormone metabolism was analyzed via LC-MS/MS.The spectrophotometric methods specified in the kits were employed to determine the physicochemical indicators associated with plant carbon and nitrogen metabolism,antioxidant capacity,photosynthesis,soil nutrients and metabolic cycling,and total phenolic acid content.Conventional methods were used to measure growth indicators and disease incidence of A.sinensis.HPLC was employed to determine the content of active components in A.sinensis roots.The correlation analysis was performed to reveal the indicators associated with the yield and quality of A.sinensis.[Results]Foliar application of R.palustris significantly increased the content of trans-zeatin riboside,dihydrozeatin-O-glucoside riboside,trans-zeatin-O-glucoside,dihydrozeatin-7-glucoside,trans-zeatin,and 1-aminocyclopropane carboxylic acid(ACC)(P<0.05).Compared with the control,the R.palustris group showed a 12%increase in chlorophyll content,a 20%increase in superoxide anion scavenging rate,a 6%increase in ABTS radical scavenging capacity,a 16%increase in total phenol content,and a 25%decrease in soil total phenolic acid(TPA)content(P<0.05).In addition,the treatment with R.palustris increased the activities of five enzymes,including glycolate oxidase and glutamine synthetase(P<0.05).The treatment increased the plant height,main root length,and hundred-plant weight of A.sinensis by 27%,21%,and 10%,respectively,decreased the incidence of root rot by 24%(P<0.05),and increased the ferulic acid and coniferyl ferulate content by 11%and 36%(P<0.05),respectively(P<0.05).Total phenolic acids showed a positive correlation with root rot incidence and a negative correlation with hundred-plant weight.ACC exhibited a negative correlation with root rot incidence and a positive correlation with hundred-plant weight.Ferulic acid and coniferyl ferulate demonstrated extremely significant positive correlations with ACC and trans-zeatin riboside.[Conclusion]R.palustris primarily degrades phenolic autotoxins and enhances reactive oxygen scavenging capacity by regulating endogenous plant hormones,particularly 1-aminocyclopropanecarboxylic acid and zeatins,thereby increasing the yield and improving the quality of A.sinensis.