文献类型：期刊文献

中文题名：生肤灵对大鼠表皮生长因子及纤维连接蛋白影响的实验研究

英文题名：Experimental Study of Shengfuling in Rat Epidermal Growth Factor and Fibronectin

作者：潘建西[1];王临青[1];张思胜[1];舒畅[2]

第一作者：潘建西

机构：[1]兰州中医骨伤科医院,兰州730010;[2]甘肃中医学院实验动物中心

第一机构：兰州中医骨伤科医院,兰州730010

年份：2012

卷号：20

期号：2

起止页码：6

中文期刊名：中国中医骨伤科杂志

外文期刊名：Chinese Journal of Traditional Medical Traumatology & Orthopedics

收录：CSTPCD

基金：兰州市科技计划项目(2010-1-68)

语种：中文

中文关键词：生肤灵;创伤修复;表皮生长因子;纤维连接蛋白;大鼠

外文关键词：Shengfuling; Wound repair; Epidermal growth factor; Fibronectin; Rat

摘要：目的:以中药生肤灵为载体,通过观察生肤灵对大鼠创面修复模型创面肉芽组织相关生长因子表达的影响,从分子水平探讨中药外用制剂促进创伤修复的机制。方法:采用经典创伤修复模型,收集创面第8天的肉芽组织,用酶联免疫分析(ELISA)检测创面中表皮生长因子(EGF)的表达及血浆纤维连接蛋白(FN)表达。结果:大鼠创面肉芽组织生长旺盛,创面肉芽组织中细胞所表达的生长因子存在差异。在同一取材实验的条件下,高低剂量组与空白对照组比较,EGF蛋白在创伤后8d存在组间差异(P<0.05)。FN表达明显高于对照组,相比差异有统计学意义(P<0.05)。结论:中药生肤灵能够通过刺激创面内细胞产生内源性生长因子进而促进创面的修复,同时减少瘢痕形成。
Objective：To explore the influence of the external application of Shengfuling on growth factor in the wound repair model and to investigate the related mechanisms.Methods：The granulation tissue of the wound fields was collected in classical wound repair model 8 days after modeling.The expression of epidermal growth factor（EGF） in tissue surface and fibronectin（FN） in blood plasma were measured by ELISA method in various groups.Results：The results showed that the expression of EGF in wound fields was different in various groups.There was a significant difference in EGF and FN expression between treatment group and control group（P0.05）.Conclusion：Shengfuling can stimulate the cells of wound fields to produce some growth factors to strengthen the function of cells,which may be the mechanism in promoting wound repair.