文献类型：期刊文献

中文题名：甘肃濒危药用植物秦艽遗传多样性的等位酶分析

英文题名：Studies on genetic diversity in endangered medicinal plant Radix Gentianae Macrophyllae of Gansu

作者：刘丽莎[1];周金霞[1];江北岸[1];王岚[1]

第一作者：刘丽莎

机构：[1]甘肃中医学院生物与生物化学教研室,兰州730000

第一机构：甘肃中医药大学基础医学院（敦煌医学研究所）

年份：2010

卷号：25

期号：9

起止页码：1476

中文期刊名：中华中医药杂志

外文期刊名：China Journal of Traditional Chinese Medicine and Pharmacy

收录：CSTPCD;;北大核心:【北大核心2008】；CSCD:【CSCD_E2011_2012】；

基金：国家中医药管理局项目(No.04-C52L23);甘肃省自然科学基金项目(No.3ZS041-A25-066)~~

语种：中文

中文关键词：甘肃;秦艽;等位酶;遗传多样性

外文关键词：Gansu; Radix Gentianae Macrophyllae; Allozyme; Genetic diversity

摘要：目的:利用等位酶分析技术对甘肃野生秦艽的遗传多样性进行分析,为甘肃秦艽的可持续发展和利用提供理论依据。方法:剪取-20℃冰镇的秦艽叶片进行酶的提取、电泳、染色及结果分析。结果:对秦艽的9个野生居群151株个体通过8个酶系统和12个酶位点的分析表明,秦艽具有较低的遗传多样性。每位点平均等位基因数(A)为1.1,多态位点百分率(P)为20.17,秦艽居群间的遗传分化系数(GST)为0.1352,说明其86.48%的遗传变异存在于居群间。结论:秦艽具有较低的遗传多样性并且在其适宜生境中完成自然更新能力较低,应采取以天水、子午岭建立就地保护区为主的综合保护策略。
Objective：To study genetic diversity in endangered medicinal plant Radix Gentianae Macrophyllae of Gansu,then to provide theoretical evidence for the sustainable development and reseanable use.Methods：The-20℃Radix Gentianae Macrophyllae iced leaves for enzyme extraction,electrophoresis,staining and analysis POPGENE software.Results：9 of Radix Gentianae Macrophyllae wild populations of 151 individuals through the eight enzyme systems and 12 enzyme loci analysis showed that lower levels of genetic diversity existed in Radix Gentianae Macrophyllae.Each point the average number of alleles locus（A）was 1.1,the percentage of polymorphic loci（P）was 20.17,and the GST was 0.1352,suggesting that 86.48%of the total genetic diversity was distributed within populations.Conclusion：Considering the relatively low levels of genetic deversity in Radix Gentianae Macrophyllae populations and the many seedlings regenerating in the smallest population,we recommend in situ conservation in Tianshui,Ziwuling to maintain viable Radix Gentianae Macrophyllae populations in their original habitats.