文献类型：期刊文献

中文题名：四神丸对溃疡性结肠炎大鼠结肠组织中核转录因子κBp65基因和蛋白表达的影响

英文题名：Effect of Sishen Pill on NF-κB p65 mRNA and Protein Expression of Colonic Mucosa in Rats with Experimental Ulcerative Colitis

作者：王燕[1];朱向东[1];段永强[1];曹燕飞[1]

第一作者：王燕

机构：[1]甘肃中医学院

第一机构：甘肃中医药大学

年份：2014

卷号：21

期号：2

起止页码：49

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2013_2014】；

基金：国家自然科学基金(81060283);甘肃省自然科学基金(1107RJZA222)

语种：中文

中文关键词：四神丸;溃疡性结肠炎;核转录因子κBp65;大鼠

外文关键词：Sishen Pill; ulcerative colitis; NF-KB p65; rats

摘要：目的观察四神丸对溃疡性结肠炎(UC)模型大鼠结肠组织中核转录因子κB p65(NF-κB p65)基因和蛋白表达的影响,探讨其作用机制。方法将实验大鼠分为空白组、模型组、四神丸组和柳氮磺胺嘧啶(SASP)组,采用TNBS/乙醇溶液灌肠法造模,四神丸组给予四神丸浸膏剂5 g/kg灌胃,SASP组给予0.3 g/kg SASP灌胃,空白组和模型组灌服等体积生理盐水。肉眼观察各组大鼠结肠大体形态损伤并评分,采用免疫组化和反转录法检测NF-κB p65蛋白和基因表达水平。结果肉眼观察,模型组大鼠结肠组织黏膜层可见炎症和溃疡形成,与空白组比较,差异有统计学意义(P<0.05)。模型组大鼠结肠组织NF-κB p65基因和蛋白表达均高于空白组,差异有统计学意义(P<0.01);四神丸组NF-κB p65基因和蛋白表达均较模型组降低,差异有统计学意义(P<0.05,P<0.01)。结论四神丸对UC具有治疗作用,其机制可能与激活NF-κB信号通路有关。
Objective To observe the influence of Sishen Pill on the NF-KB p65 mRNA and protein expressions of colonic mucosa in rats with experimental ulcerative colitis （UC）, and identify its underlying mechanism of action. Methods The experimental rats were divided into blank group, model group, Sishen Pill group and SASP group. The models were prepared by TNBS/ethanol enema. Sishen Pill group was intragastrically administrated by Sishen Pill extract 5 g/kg, SASP group by SASP 0.3 g/kg, and blank group and model group by equal volume of normal saline. The morphological injury of colonic mucosa was observed and scored with the naked eyes, and NF-KB p65 gene and protein expression were detected by RT-PCR and immunohistochemical method. Results Inflammation and ulceration on the colonic mucous membrane were found in the model group by naked eyes, and had significant difference with the blank group （P〈0.05）. The relative expression amount of NF-KB p65 gene and protein of colonic tissues were increased in the model group compared with the blank group （P〈0.01）. Compared with the model group, the relative expression amount of NF-KB p65 gene and protein in Sishen Pill group were decreased （P〈0.05, P〈0.01）. Conclusion Sishen Pill has effect for treating UC, which is probably related to the activation of NF-KB signal transduction pathway.