文献类型：期刊文献

英文题名：Non-SMC condensin I complex subunit H promotes cell proliferation and inhibits cell apoptosis of clear cell renal cell carcinoma by activating the PI3K/AKT pathway

作者：Ha, Hualan[1,2];Wang, Jieneng[3];Zhang, Xingxing[1];Du, Yuelin[1];Xiong, Wei[1];Li, Sheng[2,5];Shang, Panfeng[1,4]

第一作者：Ha, Hualan

通信作者：Shang, PF[1];Li, S[2];Shang, PF[3];Li, S[4]

机构：[1]Lanzhou Univ, Gansu Nephro Urol Clin Ctr, Key Lab Urol Dis Gansu Prov, Dept Urol,Inst Urol,Hosp 2, 82 Cui Ying Gate, Lanzhou 730030, Peoples R China;[2]Gansu Univ Tradit Chinese Med, Peoples Hosp Lanzhou City 1, Clin Med Coll 2, 1 Wujiayuan West St, Lanzhou 730050, Peoples R China;[3]Lanzhou Univ, Hosp 1, Lanzhou, Peoples R China;[4]Lanzhou Univ Second Hosp, Key Lab Urol Dis Gansu Prov, Gansu Nephrourol Clin Ctr, Dept Urol,Inst Urol, 82 Cui Ying Gate, Lanzhou 730030, Gansu, Peoples R China;[5]First Peoples Hosp Lanzhou City, Dept Cardiovasc Med, 1 Wujiayuan West St, Lanzhou City 730050, Gansu Prov, Peoples R China

第一机构：Lanzhou Univ, Gansu Nephro Urol Clin Ctr, Key Lab Urol Dis Gansu Prov, Dept Urol,Inst Urol,Hosp 2, 82 Cui Ying Gate, Lanzhou 730030, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Gansu Nephro Urol Clin Ctr, Key Lab Urol Dis Gansu Prov, Dept Urol,Inst Urol,Hosp 2, 82 Cui Ying Gate, Lanzhou 730030, Peoples R China;[2]corresponding author), Gansu Univ Tradit Chinese Med, Peoples Hosp Lanzhou City 1, Clin Med Coll 2, 1 Wujiayuan West St, Lanzhou 730050, Peoples R China;[3]corresponding author), Lanzhou Univ Second Hosp, Key Lab Urol Dis Gansu Prov, Gansu Nephrourol Clin Ctr, Dept Urol,Inst Urol, 82 Cui Ying Gate, Lanzhou 730030, Gansu, Peoples R China;[4]corresponding author), First Peoples Hosp Lanzhou City, Dept Cardiovasc Med, 1 Wujiayuan West St, Lanzhou City 730050, Gansu Prov, Peoples R China.|[10735]甘肃中医药大学;

年份：2025

卷号：14

期号：8

起止页码：2153

外文期刊名：TRANSLATIONAL ANDROLOGY AND UROLOGY

收录：;Scopus(收录号：2-s2.0-105014212134);WOS:【SCI-EXPANDED(收录号:WOS:001573220200005)】；

基金：This study was funded in China by the Natural Science Foundation of Gansu Province (No. 25JRRA1042) for the project "NCAPH activates the PI3K/mTORC2/AKT pathway to promote proliferation, invasion, and metastasis in clear cell renal cell carcinoma", Lanzhou Guiding Science and Technology Development Plan Project (No. 2024-9-182) for the project "Expression of NCAPH Molecule in Clear Cell Renal Cell Carcinoma and Its Relationship with Prognosis", and the Lanzhou Municipal Health Industry Research Project (No. A2024010) for the project "Mechanistic study of NCAPH mediating immune microenvironment in tumour bone metastasis through modulation of CAF subpopulation".

语种：英文

外文关键词：Non-SMC condensin I complex subunit H (NCAPH); clear cell renal cell carcinoma (ccRCC); PI3K/AKT

摘要：Background: Clear cell renal cell carcinoma (ccRCC) is a common cancer worldwide, frequently linked to unfavorable outcomes. The non-SMC condensin I complex subunit H (NCAPH) protein, one of the components of the non-structural maintenance of chromosomes (SMC) condensin I complex, plays a crucial part in regulating this complex, which is instrumental in the progression and advancement of various malignancies. Nonetheless, the significance of NCAPH in ccRCC is still not fully understood. This investigation was conducted to explore its potential impacts on ccRCC. Methods: This investigation employed publicly available resources, such as The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases, to evaluate the differential expression of NCAPH in ccRCC tumour tissues in comparison to corresponding normal tissues. Additionally, the study investigated the relationship between the prognostic model predicting overall survival (OS) and the advancement of ccRCC. An analysis was conducted on the genes expressed differently between groups with high and low NCAPH levels, followed by Gene Set Enrichment Analysis (GSEA) to gain further insights. Moreover, the presence of immune cell types within the context of NCAPH was also investigated. In addition to analyses from publicly available databases, assessments employing quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot techniques were utilized to determine the levels of expression of NCAPH in ccRCC cell lines and tissue samples. Cell lines with stable NCAPH knockdown were created to further investigate its functional role. To evaluate cell growth, colony formation assays and Cell Counting Kit-8 (CCK-8) tests were performed. The analysis of the cell cycle and apoptosis was carried out using flow cytometry. Additionally, Western blot techniques were conducted to determine the levels of expression of proteins associated with apoptosis, cell cycle regulation, and the PI3K/AKT signaling pathway. Results: An increase in NCAPH levels was observed in both tissues and cell lines derived from ccRCC. High levels of NCAPH were found to correlate with lower survival outcomes and a weakened immune response. The reduction of NCAPH levels could halt the progression of tumor cells during the G1 phase, which, in turn, greatly restricted their proliferation while promoting apoptosis. Additionally, it was shown that NCAPH could have an important role in activating the PI3K/AKT signaling pathway within ccRCC cells. Conclusions: Individuals with ccRCC who demonstrated elevated levels of NCAPH were at an increased likelihood of experiencing poor prognostic outcomes. Moreover, NCAPH was crucial for promoting cellular growth and inhibiting apoptosis by activating the PI3K/AKT signaling pathway in ccRCC, suggesting its potential utility as both a marker for prognosis and a target for therapy.