文献类型：期刊文献

中文题名：肝郁脾虚证模型大鼠红细胞和促红细胞生成素变化规律研究

英文题名：Changes of Red Blood Cell and Erythropoietin in Rat Models with Liver Depression and Spleen Deficiency Syndrome

作者：岳嘉[1];刘建鸿[2];程晓丽[2];车敏[2]

第一作者：岳嘉

机构：[1]甘肃中医学院公共卫生学院;[2]甘肃中医学院基础医学院

第一机构：甘肃中医药大学公共卫生学院

年份：2015

卷号：22

期号：3

起止页码：77

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2015_2016】；

基金：甘肃省自然科学研究基金计划(0803RJZA071)

语种：中文

中文关键词：肝郁脾虚证;红细胞;促红细胞生成素;大鼠

外文关键词：liver-depression and spleen-deficiency syndrome;red blood cell;erythropoietin;rats

摘要：目的检测不同时段肝郁脾虚证模型大鼠外周红细胞、骨髓红细胞及造血细胞调节因子,从血液系统探讨其变化规律。方法采用束缚应激+夹尾激怒+过度疲劳+饮食失节方法建立肝郁脾虚证大鼠模型,检测空白对照组和模型2、6、9周组大鼠外周红细胞、骨髓有核红细胞、干细胞因子及促红细胞生成素。结果与空白对照组比较,模型2周组大鼠红细胞压积和晚幼红细胞数量升高(P<0.05),模型6周组大鼠促红细胞生成素降低(P<0.01),模型9周组大鼠红细胞计数、血红蛋白含量、红细胞压积和促红细胞生成素降低(P<0.05);与模型2周组比较,模型6、9周组大鼠红细胞计数、血红蛋白含量、红细胞压积、早幼红细胞比例、晚幼红细胞比例和促红细胞生成素降低(P<0.05,P<0.01),模型9周组有核红细胞比例降低(P<0.05);干细胞因子差异无统计学意义。结论肝郁脾虚证大鼠遭受应激刺激时虽然短期能使红细胞的数量增加以对抗伤害因素作用,但长期作用会导致机体贫血,可能与促红细胞生成素减少有关。
Objective To test red cells of bone marrow and hematopoietic cells of rats with liver depression and spleen deficiency syndrome in different time period;To discuss the change rules in the blood system. Methods Rat models with liver depression and spleen deficiency syndrome were made by composite method of anger by restraint stress, clipping tails, over fatigue, and abnormal diet. The hemogram, red cells of bone marrow, marrow nucleated red blood cells, stem cell factor, and erythropoietin of male rats in normal group and model group were detected at 2nd, 6th and 9th weeks. Results Compared with normal group, hematokrit and metarubricyte in model group of 2 weeks was higher in the model group （P〈0.05）;erythropoietin in model group of 6 weeks decreased （P〈0.01）;red cell counts, red protein level, hemoglobin, hematokrit, and erythropoietin in model group of 9 weeks were lower （P〈0.05）;red cell counts, hemoglobin, hematokrit, prorubricyte, metarubricyte, and erythropoietin in model group of 6 and 9 weeks were significantly lower than those in model group of 2 weeks （P〈0.05, P〈0.01）. The number of marrow nucleated red blood cells in model group of 2 weeks decreased （P〈0.05）. Stem cell factor showed no significant difference. Conclusion The rats with liver depression and spleen deficiency syndrome can increase the number of red blood cells in a short time when they suffer from stress stimulation, so that they can fight the damage factors. But long-term stress stimulation will lead the rats to anemia, which may be associated with the reduction of erythropoietin.