文献类型：期刊文献

中文题名：温通针法对过敏性鼻炎大鼠血清免疫球蛋白E、白介素-1β、肿瘤坏死因子-α含量的影响

英文题名：Effect of Warm Acupuncture on the Levels of Serum Immunoglobulin E,Interleukin-1β and Tumor Necrosis Factor-αin Rats with Allergic Rhinitis

作者：郑先丽[1];田永萍[1];雒海燕[1];赵耀东[2];刘翔毅[1];姜影[1];马承旭[1];王明娟[1];刘敏[1]

第一作者：郑先丽

机构：[1]甘肃中医药大学附属医院针灸中心;[2]甘肃中医药大学,兰州730000

第一机构：甘肃中医药大学第二附属医院

年份：2018

卷号：43

期号：1

起止页码：34

中文期刊名：针刺研究

外文期刊名：Acupuncture Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；PubMed;

基金：甘肃郑氏针法学术流派传承工作室开放基金(No.LP 0128060-KFJJ 05);甘肃省科技支撑计划项目(No.1304FKCA 096);甘肃省自然科学基金项目(No.096RJZA 043);甘肃省重点中医药科研项目(No.GZK-2010-Z 12)

语种：中文

中文关键词：温通针法;过敏性鼻炎;行为学;免疫球蛋白E

外文关键词：Warm acupuncture; Allergic rhinitis; Behavior; Immunoglobulin E

摘要：目的:观察温通针法对过敏性鼻炎(AR)大鼠行为学和血清中免疫球蛋白(Ig)E、白介素(IL)-1β、肿瘤坏死因子(TNF)-α含量的影响,探讨温通针法治疗AR过敏反应的作用机制。方法:Wistar大鼠随机分为空白对照组、模型组、药物组和温通针法组,每组10只。采用腹腔注射基础致敏和鼻腔滴入局部触发的方式复制AR模型。药物组大鼠每天予以丙酸氟替卡松喷鼻,每天1次,温通针法组予温通针法针刺"风池""印堂""迎香",每日1次,每次每穴操作60s,两组均治疗10d。采用行为学评分方法评估大鼠行为学变化,酶联免疫吸附测定法检测各组大鼠血清中IgE、IL-1β、TNF-α的含量。结果:行为学评分结果显示,模型组评分显著高于空白对照组(P<0.05);治疗第3天药物组及第7、10天药物组、温通针法组行为学评分均低于模型组(P<0.05);治疗第10天温通针法组行为学评分显著低于药物组(P<0.05)。与空白对照组相比,模型组大鼠血清中IgE、IL-1β、TNF-α含量均显著增加(P<0.01);与模型组相比,温通针法组、药物组大鼠血清中IgE、IL-1β、TNF-a含量均显著降低(P<0.05,P<0.01);与药物组比较,温通针法组IgE、IL-1β、TNF-α均显著降低(P<0.05,P<0.01)。结论:AR发作后血清中过敏介质IgE和炎性因子IL-1β、TNF-α含量升高,温通针法可以改善AR模型大鼠发作期的症状,对AR有明显的治疗作用,其对症状的治疗作用机制可能与抑制上游过敏介质IgE和炎性因子IL-1β、TNF-α的生成有关。
Objective To observe the effect of warm acupuncture on behavior and contents of serum immunoglobulin E （IgE）, interleukin-1β（IL-1β） and tumor necrosis factor-α（TNF-α） in allergic rhinitis（AR） rats, so as to explore its mechanism underlying improving AR. Methods Forty Wistar rats were randomly divided into four groups： control group, model group, medication group and warm acupuncture group（10 rats/group）. The AR model was established by intraperitoneal injection of sensitization and nasal drip. The rats in the medication group were given fluticasone propionate nasal spray, daily for 10 days. Warm acupuncture was applied to ＂Fengchi＂（GB 20）, ＂Yintang＂（GV 29）, ＂Yingxiang＂（LI 20） for 60 seconds, once daily for 10 days. Behavioral scores were used to evaluate behavioral changes in rats. Enzyme linked immunosorbent assay （ELISA） was used to detect the expression levels of serum IgE, IL-1β and TNF-α. Results Behavioral scores of the model group were significantly higher than those of the control group 0, 3, 7 and 10 days after modeling （P〈0.05）. After treatment, the behavioral scores of medication group and the warm acupuncture group were lower than those of the model group （ P〈0.05）, and the score was more lower in the warm acupuncture group than in the medication group （P〈0.05）. Compared with the control group, the levels of serum IgE, IL-1β and TNF-α in the model group were all increased （P〈0.01）, while the levels of serum IgE, IL-1β and TNF-α were decreased in the medication and warm acupuncture groups after treatment in comparison with the model group （P〈0, 05,P〈0.01）. Compared with the medication group, the levels of serum IgE, IL-1β and TNF-α were significantly lower in the warm acupuncture group （P〈0.05, P〈0.01）. Conclusion The expression levels of IgE, IL-1β and TNF-α were elevated in serum after AR attack. Warm acupuncture can improve the symptoms of AR rats, which may be associated to its effect in inhibiting the expression of serum IgE, IL-1β and TNF-α.