文献类型：期刊文献

中文题名：红芪多糖对免疫抑制大鼠免疫功能与细胞因子的影响

英文题名：Effect of Radix hedysari polysaccharide on the immune function and cytokines of immune suppression induced by cyclophosphamide in rat

作者：颜春鲁[1];李钦[2];姚贞宇[3];刘永琦[1]

第一作者：颜春鲁

机构：[1]甘肃中医药大学甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室,兰州730000;[2]甘肃省中医学校,兰州730050;[3]兰州市第一人民医院,兰州730050

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2017

卷号：33

期号：13

起止页码：1233

中文期刊名：中国临床药理学杂志

外文期刊名：The Chinese Journal of Clinical Pharmacology

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD2017_2018】；

基金：甘肃省中医药管理局科研基金资助项目(GZK-2015-45)

语种：中文

中文关键词：红芪多糖;免疫抑制;免疫功能;细胞因子

外文关键词：Radix hedysari polysaccharide; immunosuppression; immunoglobulin; cytokines

摘要：目的观察红芪多糖对免疫抑制大鼠免疫功能与细胞因子的影响。方法SD大鼠腹腔注射环磷酰胺80 mg·kg^(-1),1次/天,连用3 d,构建免疫抑制模型大鼠。从造模成功后的模型大鼠中选取20只,按性别、体重随机分为2组(每组10只):模型组和实验组;另设10只健康大鼠为空白组。实验组每只大鼠按100 mg·kg^(-1)·d-1剂量灌服相应药物,模型组及空白组每只大鼠灌服等体积的蒸馏水,1次/天,连续7 d。给药7 d后,各组大鼠禁食8 h,股动脉取血,用酶联免疫法检测血清中免疫球蛋白Ig G、Ig M及Ig A的含量;用同法检测血清中细胞因子肿瘤坏死因子-α(TNF-α)、γ-干扰素(IFN-γ)、白细胞介素-2(IL-2)和IL-4的含量。结果给药后,空白组、模型组与实验组大鼠的Ig G分别是(0.98±0.50),(0.28±0.18),(0.60±0.28)μg·m L^(-1);这3组的Ig M分别是(365.21±26.85),(267.03±47.89),(286.22±30.03)μg·m L^(-1);这3组的Ig A分别是(79.52±9.30),(57.88±10.44),(69.19±14.18)μg·m L^(-1)。与空白组比较,模型组差异均有统计学意义(均P<0.05);与模型组比较,实验组差异有统计学意义(均P<0.05)。空白组、模型组与实验组大鼠的TNF-α分别是(39.52±5.28),(70.14±6.68),(51.16±5.60)pg·m L^(-1);这3组的IFN-γ分别是(24.31±5.86),(10.88±3.17),(15.52±3.92)pg·m L^(-1);这3组的IL-2分别是(261.51±46.20),(153.7±34.96),(185.84±17.74)pg·m L^(-1);这3组的IL-4分别是(18.91±2.56),(9.32±3.86),(15.23±2.75)pg·m L^(-1)。与空白组比较,模型组差异有统计学意义(均P<0.05);与模型组比较,实验组差异有统计学意义(均P<0.05)。结论红芪多糖能提高环磷酰胺所致免疫抑制模型大鼠的免疫功能。
Objective To observe the effect of Radix hedysari polysaccharide（ RHP） on the immune function and cytokines of immune suppression induced by cyclophosphamide in rats. Methods Sixty SD rats were treated with intraperitoneal injection of cyclophosphamide（ CTX）,each time 80 mg·kg^（-1）,1 times/day,with 3 d. After the success of the model group intervention treatment,the rats was divided into 3 groups：blank group,model group and experimental group. The rats in experimental group was treated with the dose of 100 mg·kg^（-1）·d-1 RHP,and the rats in the blank group and the model group were fed with distilled water of equal volume,1 times/day,administrating 7 d. Drug delivery in 7 days,rats in groups fasting 8 hours,femoral artery blood. The serum levels of immunoglobulin Ig G,Ig M and Ig A were detected byenzyme league immune method. The serum content of immune cell factor of tumor necrosis factorα（ TNF-α）,interferon-γ（ IFN-γ）,interleukin-2（ IL-2） and IL-4 were detected by enzyme league immune method. Results After administration,IgG in blank group,model group and experimental group were（ 0. 98 ± 0. 50）,（ 0. 28 ± 0. 18）,（ 0. 60 ± 0. 28） μg·mL^（-1）; IgM in the three groups were（ 365. 21 ± 26. 85）,（ 267. 03 ± 47. 89）,（ 286. 22 ± 30. 03）μg·m L^（-1）; IgA in the three groups were（ 79. 52 ± 9. 30）,（ 57. 88 ± 10. 44）,（ 6 9. 19 ± 14. 18） μg·mL^（-1）. Compared with blank group,the difference in model group was statistically significant（ all P〈0. 05）. Compared with model group,the difference in experimental group was statistically significant（ all P〈0. 05）. After administration,TNF-αin blank group,model group and experimental group were（ 39. 52 ± 5. 28）,（ 70. 14 ± 6. 68）,（ 51. 16 ± 5. 60）pg·mL^（-1）; IFN-γ in the three groups were（ 24. 31 ± 5. 86）,（ 10. 88 ± 3. 17）,（ 15. 52 ± 3. 92） pg·m L^（-1）; IL-2 in the three groups were（ 261. 51 ± 46. 20）,（ 153. 7 ± 34. 96）,（ 185. 84 ± 17. 74） pg·mL^（-1）; IL-4 in the three groups were（ 18. 91 ± 2. 56）,（ 9. 32 ± 3. 86）,（ 15. 23 ± 2. 75） pg · mL^（-1）. Compared with blank group,the difference in model group was statistically significant（ all P〈0. 05）. Compared with model group,the difference in experimental group was statistically significant（ all P〈0. 05）. Conclusion RHP can improve the immunosuppression caused by cyclophosphamide model immune function in rats.