文献类型：期刊文献

中文题名：反相高效液相色谱法测定藏药薰倒牛中木犀草素及槲皮素含量

英文题名：Determination of luteolin and quercetin in Biebersteinia heterostemon Maxim. by RP-HPLC

作者：刘娟娟[1];刘雪枫[1];陈晖[2];赵慧巧[1];卢年华[1];景明[1]

第一作者：刘娟娟

机构：[1]甘肃中医药大学药学院;[2]甘肃中医药大学理科教学部

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2015

卷号：0

期号：6

起止页码：23

中文期刊名：甘肃中医学院学报

外文期刊名：Journal of Gansu College of Traditional Chinese Medicine

基金：兰州市社会发展科技计划项目(2012-1-57)

语种：中文

中文关键词：藏药熏倒牛;木犀草素;槲皮素;反相高效液相色谱;含量测定

外文关键词：Biebersteinia heterostemon Maxim.;;luteolin;;quercetin;;RP-HPLC;;assaying

摘要：目的建立藏药熏倒牛中木犀草素及槲皮素的含量测定方法。方法采用反相高效液相色谱（RP-HPLC）法,以SB-C18为填充剂,甲醇-0.4%磷酸水溶液（48∶52）为流动相,检测波长为350 nm,建立了一种可同时测定藏药熏倒牛药材中木犀草素与槲皮素含量的方法。结果木犀草素回归方程为Y=8.97×107X+1.06×105（R=0.999 9,n=5）,线性范围为6.0836.48μg/m L,平均加样回收率为97.69%,RSD为1.56%;槲皮素回归方程为Y=6.91×107X+7.77×103（R=0.999 8,n=5）,线性范围为2.5619.2μg/m L,平均加样回收率为98.31%,RSD为1.41%。结论该方法简便、快速、准确,可用于同时测定熏倒牛中木犀草素、槲皮素的含量及药材质量控制。
Objective To establish a method for determining the content of luteolin and quercetin in Biebersteinia heterostemon Maxim.( traditional Tibetan medicine). Methods The content of luteolin and quercetin in Biebersteinia h eterostemon Maxim. was determined by RP-HPLC. The mobile phase was methanol: phosphoric acid solution( 0.4%) at 48 ∶ 52,and UV detection wavelength was set at 350 nm. A method was established for simultaneously determining the content of luteolin and quercetin in Biebersteinia heterostemon Maxim.. Results The equation of l inear regression of luteolin was Y = 8.97×10~7X +1.06×10~5( R=0.999 9,n=5),the linear range was 6.08 to 3 6.48 μg/m L,the average sample recovery rate was 97.69%,RSD was 1.56%. The equation of linear regression of quercetin was Y =6.91×10~7X +7.77×10~3( R=0.999 8,n=5),the linear range was 2.56 to 19.2 μg/m L. The ave-rage sample recovery rate was 98.31%,RSD was 1.41%. Conclusion The method is simple,rapid and accurate,which can determine the content of luteolin and quercetin in Biebersteinia heterostemon Maxim. simultaneously and control medicinal materials quality.