文献类型：期刊文献

英文题名：Realgar Nanoparticles Inhibit Migration, Invasion and Metastasis in a Mouse Model of Breast Cancer by Suppressing Matrix Metalloproteinases and Angiogenesis

作者：Xi Xiaoxia[1];Sun Jing[1];Xi Dongbin[2];Tian Yonggang[1];Zhang Jingke[1];Zhang Yanying[3];Wei Hulai[1]

第一作者：Xi Xiaoxia

通信作者：Zhang, YY[1];Wei, HL[2]

机构：[1]Lanzhou Univ, Sch Basic Med Sci, Key Lab Preclin Study New Drugs Gansu Prov, Lanzhou, Peoples R China;[2]Peoples Hosp Jiuquan City, Gen Surg Dept, Jiuquan, Peoples R China;[3]Gansu Univ Chinese Med, Lab Anim Ctr, 35 Ding Xi East Rd, Lanzhou, Gansu, Peoples R China

第一机构：Lanzhou Univ, Sch Basic Med Sci, Key Lab Preclin Study New Drugs Gansu Prov, Lanzhou, Peoples R China

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Lab Anim Ctr, 35 Ding Xi East Rd, Lanzhou, Gansu, Peoples R China;[2]corresponding author), Lanzhou Univ, Sch Basic Med Coll, 199 Dong Gang West Rd, Lanzhou, Gansu, Peoples R China.|[10735]甘肃中医药大学;

年份：2020

卷号：17

期号：2

起止页码：148

外文期刊名：CURRENT DRUG DELIVERY

收录：;Scopus(收录号：2-s2.0-85081945418);WOS:【SCI-EXPANDED(收录号:WOS:000519987900006)】；

基金：This study was supported in part by grants from the Natural Science Fund of Gansu Province (17JR5RA192) and the Fundamental Research Funds for the Central Universities (lzujbky-2015-157, lzujbky-2015-192).

语种：英文

外文关键词：Nano-realgar; breast cancer; xenograft; metastasis; MMPs; angiogenesis

摘要：Background: Realgar, a traditional Chinese medicine. has shown antitumor efficacy in several tumor types. We previously showed that realgar nanoparticles (nano-realgar) had significant ant-ileukemia, anti-lung cancer and anti-liver cancer effects. In addition, the anti-tumor effects of nano-realgar were significantly better than those of ordinary realgar. Objective: To explore the inhibitory effects and molecular mechanisms of nano-realgar on the migration, invasion and metastasis of mouse breast cancer cells. Methods: Wound-healing migration assays and Transwell invasion assays were carried out to determine the effects of nano-realgar on breast cancer cell (4T1) migration and invasion. The expression levels of matrix metalloproteinase (MMP)-2 and -9 were measured by Western blot. A murine breast cancer metastasis model was established, administered nano-realgar for 32 days and monitored for tumor growth and metastasis by an in vivo optical imaging system. Finally, living imaging and hematoxylin and eosin (HE) staining were used to measure the morphology and pathology of lung and liver cancer cell metastases, respectively. Angiogencsis was assessed by CD34 immunohistochemistry. Results: Nano-realgar significantly inhibited the migration and invasion of breast cancer 4T1 cells and the expression of MMP-2 and -9. Meanwhile, nano-realgar effectively suppressed the abilities of tumor growth, metastasis and angiogenesis in the murine breast cancer metastasis model in a time- and dose-dependent manner. Conclusion: Nano-realgar significantly inhibited migration and invasion of mouse breast cancer cells in vitro as well as pulmonary and hepatic metastasis in vivo, which may be closely correlated with the downexpression of MMP-2 and -9 and suppression of tumor neovascularization.