文献类型：期刊文献

中文题名：当归补血汤含药血清对血管紧张素II诱导的心肌细胞凋亡及相关蛋白表达的影响

英文题名：Effect of Serum Containing Danggui Buxue Decoction on the Apoptosis and Related Protein Eexpression of Angiotesin II-induced Cardiomyocytes

作者：孙艳[1];孙樱丹[1];徐厚谦[1]

第一作者：孙艳

机构：[1]甘肃中医学院,兰州730000

第一机构：甘肃中医药大学

年份：2013

卷号：19

期号：19

起止页码：250

中文期刊名：中国实验方剂学杂志

外文期刊名：Chinese Journal of Experimental Traditional Medical Formulae

收录：北大核心:【北大核心2011】；

基金：甘肃省中医药管局(英才计划),编号:GZK-2010-56

语种：中文

中文关键词：当归补血汤;血管紧张素Ⅱ;心肌细胞;细胞凋亡

外文关键词：Danggui Buxue decoction; angiotesin II ; cardiomyocyte ; apoptosis

摘要：目的:探讨当归补血汤含药血清对血管紧张素Ⅱ(Ang Ⅱ)诱导的心肌细胞凋亡及相关蛋白表达的影响。方法:应用血清药理学方法制备含药血清。体外培养心肌细胞,收集对数期细胞分为4组:空白对照组、AngⅡ(10-7mol·L-1)组、缬沙坦(10-6mol·L-1)组、10%当归补血汤含药血清(0.84 g·L-1)组,共同作用48 h后,用MTT法测定细胞活性、单细胞电泳法检测细胞DNA损伤情况、激光共聚焦检测线粒体膜电位,Western blotting检测细胞凋亡相关蛋白p53,Caspase-3表达变化。结果:与空白对照组相比,Ang Ⅱ组心肌细胞活力(A)(0.159±0.024)、线粒体膜电位(170.19±16.15)Δψm明显降低(P<0.05),并且心肌细胞DNA损伤,p53(0.497±0.029)、Caspase-3(0.509±0.041)蛋白表达显著升高(P<0.05);与Ang II组相比,当归补血汤含药血清组心肌细胞活性(A)(0.197±0.035)、线粒体膜电位(242.39±18.27)Δψm明显升高(P<0.05),心肌细胞DNA损伤、p53(0.387±0.020),Caspase-3(0.385±0.029)蛋白表达显著降低(P<0.05)。结论:当归补血汤可抑制心肌细胞凋亡,其机制可能与调节线粒体通路有关。
Objective： To investigate effect of serum containing Danggui Buxue decoction on the apoptosis and related protein expression of angiotensin II （Ang II） -induced myocardial. Method： Danggui Buxue decoction was prepared by serum pharmacology method. The cardiomyocytes in the logarithmic growth phase were divided into 4 groups： control group, Ang II （10-7 mol·L^-1） group, valsartan （1 x 10-6mol ·L^-1） group and 10% medicated serum （0. 84 g ·L^-1） group, each group would be detected after 48 hours. Cell activity was measured by MTT method. Cardiomyocytes DNA damage was detected by Single~cell gel electrophoresis. Laser confocal scanning microscopy was used to detect the mitochondrial membrane potential. Western blotting was used to study the changes of p53 protein and Caspase-3 protein. Result： The myocardial cell activity （A） （0. 159 ±0. 024） and mitochondrial membrane potential （△ψm） （170. 19 ±16. 15 ） in Ang II group were significantly lower than those of blank control group （P 〈 0.05）, but myocardial cell DNA damage, p53 （0. 497 ±0. 029） and Caspase-3 （0. 509 ±0. 041 ） protein expression were obviously higher than that of blank control group （P 〈 0.05）; the myocardial cell activity （A） （0. 197 ±0. 035 ） and mitochondrial membrane potential （△ψm） （242.39 ± 18.27） in Danggui Buxue decoction containing serum group were significantly higher than those of Ang II group （P 〈0.05）, but myocardial cell DNA damage, p53 （0.387 ±0.020） and Caspase-3 （0.385 ±0. 029） protein expression was obviously lowdr than that of Ang II group （P 〈 0.05）. Conclusion： Danggui Buxue decoction could inhibit the apoptosis of Ang II-induced cardiomyocyte hypertrophy. It may be related with mitochondria-dependent apoptotic pathway.