文献类型：期刊文献

中文题名：基于新型溶剂系统筛选的高速逆流色谱分离油橄榄叶中的多酚类化合物

英文题名：High-speed countercurrent chromatography based on screening of new solvent systems in separation of polyphenols compounds from olive leaves

作者：王玉[1,2];夏鹏飞[1];杨雪[1];段文达[1];全凯军[2];黄新异[2];邸多隆[1,2];赵磊[1]

第一作者：王玉

机构：[1]甘肃中医药大学药学院,甘肃730000;[2]中国科学院兰州化学物理研究所中国科学院西北特色植物资源化学重点实验室甘肃天然药物重点实验室

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2019

卷号：42

期号：9

起止页码：766

中文期刊名：北京中医药大学学报

外文期刊名：Journal of Beijing University of Traditional Chinese Medicine

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：国家自然科学基金青年项目(No.81660577);甘肃省中医药管理局科研课题项目(No.G2KLP-2014-7);甘肃省中医药管理局科研项目(No.G2K-2014-77);甘肃省道地药材质量标准化技术研究与推广工程实验室开放基金项目(No.ddyc-2016-04)~~

语种：中文

中文关键词：新型溶剂系统;高速逆流色谱;油橄榄叶;分离

外文关键词：new solvent system;HSCCC;olive leaves;separation

摘要：目的采用HEMW(正己烷-乙酸乙酯-甲醇-水)系统组成基于9×9表新型溶剂系统筛选的方法应用于高速逆流色谱(HSCCC)分离油橄榄叶中的多酚类化合物橄榄苦苷、木犀草素-4’-O-β-D-葡萄糖苷和羟基酪醇。方法通过测定目标物在4种溶剂体系中的分配系数K值并在LogK值和溶剂系统数之间构建相关的线性关系,筛选出最佳溶剂系统HEMW(1.2∶8.8∶1.7∶8.3,V/V)进行HSCCC分离油橄榄叶提取物并优化了流速、转速及进样量等参数。结果计算所得HEMW(1.2∶8.8∶1.7∶8.3,V/V)为溶剂系统,流速为1.5 mL/min、转速为950 r/min、进样浓度为20 g/L从500 mg油橄榄叶提取物中分离得到45.8 mg橄榄苦苷、17.7 mg木犀草素-4’-O-β-D-葡萄糖苷及6.8 mg羟基酪醇,且3种化合物纯度均大于90%。结论本研究表明9×9表溶剂系统筛选应用于HSCCC分离天然产物领域中具有可行性和高效性,并为HSCCC溶剂系统快速筛选及提高其从复杂体系中分离制备目标化合物的效率提供了技术支持。
Objective A novel 9×9 map-based solvent selection strategy for a system composed of hexane-ethyl acetate-methanol-water(HEMW)was applied to high-speed countercurrent chromatography(HSCCC)separation of polyphenols oleuropein,luteolin-4’-O-β-D-glucoside and hydroxytyrosol from olive leaf extracts.Methods The sweet solvent system HEMW(1.2∶8.8∶1.7∶8.3,V/V)was selected by determining the distribution coefficient K values of the target compounds in four different solvent systems and establishing linear relationships between the Log K values and the number of solvent systems.The sweet solvent system HEMW(1.2∶8.8∶1.7∶8.3,V/V)was then used to separate olive leaf extracts by HSCCC,and the parameters of flow rate,rotational speed and injection volume were optimized.Results 45.8 mg oleuropein,17.7 mg luteolin-4’-O-β-D-glucoside and 6.8 mg hydroxytyrosol were obtained from 500 mg olive leaf extracts by using the sweet solvent system HEMW(1.2∶8.8∶1.7∶8.3,V/V)with flow rate at 1.5 mL/min,rotation speed at 950 r/min and injection concentration at 20 g/L,and the purity of each of the three compounds obtained was more than 90%.Conclusion This study demonstrates the feasibility and efficiency of 9×9 map-based solvent selection strategy in the field of HSCCC separation of natural products,and provides technical support for rapid screening of HSCCC solvent system and improving the efficiency of HSCCC separation and preparation of target compounds in complex systems.