文献类型：期刊文献

中文题名：唐古特大黄抗炎成分的纯化工艺优化及其抗炎作用研究

英文题名：Optimization of the purification process for anti-inflammatory constituents of Rheum tanguticum and study on its anti-inflammatory effects

作者：叶蕾蕾[1];张成园[1];翟婷[1];邵晶[1];朱昕宇[1];李阳[1];郭玫[1]

第一作者：叶蕾蕾

机构：[1]甘肃中医药大学甘肃省高校中(藏)药化学与质量研究省级重点实验室,甘肃兰州730000

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2024

卷号：39

期号：5

起止页码：503

中文期刊名：华西药学杂志

外文期刊名：West China Journal of Pharmaceutical Sciences

收录：CSTPCD;;北大核心:【北大核心2023】；CSCD:【CSCD_E2023_2024】；

基金：国家自然基金联合基金重点项目(U21A20412);甘肃省教育厅“双一流”科研重点项目(GSSYLXM-05)。

语种：中文

中文关键词：唐古特大黄;抗炎活性成分;含量测定;大孔吸附树脂;分离;纯化工艺;抗炎活性

外文关键词：Rheum tanguticum;Anti-inflammatory active constituents;Content determination;Macroporous adsorption resin;Separate;Purification process;Anti-inflammatory activity

摘要：目的优化唐古特大黄抗炎成分组(RTAC)的纯化工艺及其抗炎作用研究。方法以唐古特大黄中的10种抗炎活性成分作为抗炎活性成分组,包括番泻苷B、芒柄花苷、番泻苷A、大黄素-8-O-葡萄糖苷、芒柄花素、芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚等。采用Agilent TC-C_(18)色谱柱(250 mm×4.6 mm,5μm),以0.1%甲酸溶液-甲醇为流动相,梯度洗脱,流速1 mL·min^(-1),柱温30℃,进样量10μL,检测波长280 nm。用溶剂法和大孔吸附树脂提取RTAC并进行分离纯化,考察其抗炎活性。结果RTAC中的10种活性成分主要存在于二氯甲烷和水饱和正丁醇部位;水饱和正丁醇部位用大孔吸附树脂进一步纯化后,RTAC的含量从6.27%升至40.08%。纯化后的RTAC各剂量均可显著降低小鼠耳、足、棉球肉芽的肿胀度,且高剂量组的效果显著优于纯化前的唐古特大黄甲醇提取物组。结论所用方法可用于分离纯化唐古特大黄的抗炎活性成分,为药材的的开发利用提供了依据。
OBJECTIVE To optimize the purification process of anti-inflammatory constituents group from Rheum tanguticum(RTAC)and to study its anti-inflammatory effects.METHODS The 10 kinds including sennoside B,ononin,sennoside A,emodin 8-O-glucoside,mangostensin,aloe emodin,rhein,emodin,chrysophanol,and emodin methyl ether were used as RTAC.The HPLC analysis was performed on an Agilent TC-C_(18)chromatography column(250 mm×4.6 mm,5μm).The mobile phase consisted of 0.1%formic acid aqueous solution-methanol.The flow rate was 1 mL·min^(-1),and the column temperature was 30℃with the injection volume of 10μL and the detection wavelength of 280 nm.The extraction,separation and purification of RTAC were used by the solvent method and macroporous adsorption resin method,and the activity of RTAC was investigated.RESULTS 10 components in the RTAC of mainly existed in the dichloromethane and water saturated n-butanol parts.The water saturated n-butanol part was further purified with macroporous adsorption resin,and its mass fraction increased from 6.27%to 40.08%.The purified RTAC with each dose significantly reduced the swelling of mice ears,feet,and cotton ball granulation,and the high-dose group had significantly better effects than the pre-purified R.tanguticum methanol extracts group.CONCLUSION This method can be used to separate and purify the RTAC,providing a basis for the further development and utilization of Rheum tanguticum.