文献类型：期刊文献

英文题名：Zinc finger protein 263 promotes colorectal cancer cell progression by activating STAT3 and enhancing chemoradiotherapy resistance

作者：Du, Yadan[1,3];Chen, Yawen[1,3];Yan, Zaihua[2];Yang, Jian[3];Da, Mingxu[1,3]

第一作者：Du, Yadan

通信作者：Da, M[1];Da, M[2]

机构：[1]Gansu Univ Chinese Med, Sch Clin Med 1, Lanzhou 730000, Peoples R China;[2]North Sichuan Med Coll, Affiliated Hosp, Dept Gastrointestinal Surg 2, Nanchong 637000, Peoples R China;[3]Gansu Prov Hosp, Dept Surg Oncol, Lanzhou 730000, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Sch Clin Med 1, Lanzhou 730000, Peoples R China;[2]corresponding author), Gansu Prov Hosp, Dept Surg Oncol, Lanzhou 730000, Peoples R China.|[10735]甘肃中医药大学;

年份：2024

卷号：14

期号：1

外文期刊名：SCIENTIFIC REPORTS

收录：;Scopus(收录号：2-s2.0-85204417695);WOS:【SCI-EXPANDED(收录号:WOS:001322528400021)】；

基金：This work was supported by the National Natural Science Foundation of China (No:82160588), the Gansu Natural Science Foundation (No:21JR1RA016, No:21JR7RA598) and the Gansu Youth Natural Science Foundation (No: 21JR7RA645, No:21JR7RA646).

语种：英文

外文关键词：Colorectal cancer; Zinc finger protein 263; Signal transducer and activator of transcription 3; Radiotherapy; Chemotherapy; Epithelial-mesenchymal transition

摘要：Zinc finger protein 263 (ZNF263) is frequently upregulated in various tumor types; however, its function and regulatory mechanism in colorectal cancer (CRC) have not yet been elucidated. In this study, the expression of ZNF263 was systematically examined using data from The Cancer Genome Atlas database and samples from patients with CRC. The results indicated that high expression of ZNF263 in CRC tissues is significantly associated with tumor grade, lymph node metastasis and disant metastasis. Additionally, overexpression of ZNF263 significantly promoted the proliferation, invasion, migration, and epithelial-mesenchymal transition of CRC cells, while also increasing signal transducer and activator of transcription 3 (STAT3) expression and mRNA stability. Conversely, knockdown of ZNF263 inhibited the malignant behavior of CRC cells and decreased STAT3 expression and mRNA stability. Further mechanism studies using chromatin immunoprecipitation (CHIP) and luciferase assays verified that ZNF263 directly binds to the STAT3 promoter. Rescue experiments demonstrated that the knockdown or overexpression of STAT3 could significantly reverse the effects of ZNF263 on CRC cells. Additionally, our study found that overexpression of ZNF263 enhanced the resistance of CRC cells to the chemoradiotherapy. In summary, this study not only elucidated the significant role of ZNF263 in CRC but also proposed novel approaches and methodologies for the diagnosis and treatment of this malignancy.