文献类型：期刊文献

中文题名：基于硫酸-蒽酮法对不同产地藏药线叶龙胆多糖的含量测定

英文题名：Determination of polysaccharides content of Gentiana farreri from different producing areas based on anthrone-sulfuric acid method

作者：林丽[1,2,3];王琰[1];王福星[1];何佳研[1];张杭林[1]

第一作者：林丽

机构：[1]甘肃中医学院,甘肃兰州730000;[2]中藏药资源研究所,甘肃兰州730000;[3]甘肃省高校中戚药化学与质量研究省级重点实验室,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2014

卷号：39

期号：14

起止页码：2774

中文期刊名：中国中药杂志

外文期刊名：China Journal of Chinese Materia Medica

收录：MEDLINE(收录号：25272513);CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；PubMed;

基金：2010年甘肃中医学院大学生创新项目

语种：中文

中文关键词：硫酸-蒽酮法;线叶龙胆;多糖

外文关键词：anthrone-sulfuric acid method;Gentiana farreri;polysaccharides

摘要：研究线叶龙胆药材中多糖含量的测定方法,分析不同产地线叶龙胆药材中多糖的含量。结果表明,采用硫酸-蒽酮法,操作简便,结果准确;样品经硫酸-蒽酮显色后于620 nm处测定吸收度,在此波长处溶液的吸收度与葡萄糖含量呈良好的线性关系;线性范围0.01～0.07 g·L-1（r=0.996 7）,加样回收率99.41%,RSD 2.0%;综合考虑实验条件,确定料液比为1∶60、提取时间为50 min、乙醇浓度为4倍量（体积分数80%）。测得产自甘肃夏河的线叶龙胆中多糖质量分数为0.743%,含量最高,为线叶龙胆多糖的进一步研究奠定良好的基础。
Study a method for the detemination of the content of polysaccharides in Gentiana farreri,and analysis of the content of polysaccharides from different producing areas. The results showed that using the anthrone-sulfuric acid method,simple operation,accurate result. Sample was measured at 620 nm absorbance after anthrone-sulfuric acid color,at this wavelength,solution absorption and glucose showed a good linear relationship; The linearity was in the range of 0. 01-0. 07 g·L-1（ r =0.996 7）,The recovery rate was 99. 41%,with RSD of 2. 0%. Considering the experimental conditions,to determine the solid-liquid ratio 1∶ 60,extracting time50 min,concentration of ethanol 80%. The mass fraction of polysaccharides was the highest to reached 0. 743% in G. farreri from Gansu Xiahe. This experiment has laid a good foundation for further study on G. farreri.