文献类型：期刊文献

英文题名：Identification of Potential Prognostic Biomarkers Associated With Macrophage M2 Infiltration in Gastric Cancer

作者：Liu, Baohong[1];Ma, Xueting[1];Ha, Wei[2]

第一作者：Liu, Baohong

通信作者：Liu, BH[1]

机构：[1]Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Key Lab Vet Parasitol Gansu Prov, Lanzhou, Peoples R China;[2]Gansu Univ Tradit Chinese Med, Dept Radiol, Affiliated Hosp, Lanzhou, Peoples R China

第一机构：Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Key Lab Vet Parasitol Gansu Prov, Lanzhou, Peoples R China

通信机构：[1]corresponding author), Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Key Lab Vet Parasitol Gansu Prov, Lanzhou, Peoples R China.

年份：2022

卷号：13

外文期刊名：FRONTIERS IN GENETICS

收录：;Scopus(收录号：2-s2.0-85123950979);WOS:【SCI-EXPANDED(收录号:WOS:000890880200001)】；

基金：This work was supported by the Innovative Special Project of Agricultural Sci-Tech (Grant No. CAASASTIP-2014-LVRI-09).

语种：英文

外文关键词：gastric cancer; prognosis biomarkers; macrophage M2; weighted gene co-expression network analysis; Protein-protein interaction network

摘要：Gastric cancer is a common cancer afflicting people worldwide. Although incremental progress has been achieved in gastric cancer research, the molecular mechanisms underlying remain unclear. In this study, we conducted bioinformatics methods to identify prognostic marker genes associated with gastric cancer progression. Three hundred and twenty-seven overlapping DEGs were identified from three GEO microarray datasets. Functional enrichment analysis revealed that these DEGs are involved in extracellular matrix organization, tissue development, extracellular matrix-receptor interaction, ECM-receptor interaction, PI3K-Akt signaling pathway, focal adhesion, and protein digestion and absorption. A protein-protein interaction network (PPI) was constructed for the DEGs in which 25 hub genes were obtained. Furthermore, the turquoise module was identified to be significantly positively coexpressed with macrophage M2 infiltration by weighted gene coexpression network analysis (WGCNA). Hub genes of COL1A1, COL4A1, COL12A1, and PDGFRB were overlapped in both PPI hub gene list and the turquoise module with significant association with the prognosis in gastric cancer. Moreover, functional analysis demonstrated that these hub genes play pivotal roles in cancer cell proliferation and invasion. The investigation of the gene markers can help deepen our understanding of the molecular mechanisms of gastric cancer. In addition, these genes may serve as potential prognostic biomarkers for gastric cancer diagnosis.