文献类型：期刊文献

英文题名：Quantitative Analysis of the Multicomponent and Spectrum-Effect Correlation of the Antispasmodic Activity of Shaoyao-Gancao Decoction

作者：Xu, Yanli[1,2];Li, Chenxi[2];Chen, Ting[2];Li, Xiaochun[2];Wu, Xiaoyu[1,3,4,5];Zhang, Qili[1,3];Zhao, Lei[1,2,3,4,5]

第一作者：徐亚莉;Xu, Yanli

通信作者：Zhao, L[1];Zhao, L[2];Zhao, L[3];Zhao, L[4];Zhao, L[5]

机构：[1]Gansu Univ Chinese Med, Lanzhou 730000, Peoples R China;[2]Lanzhou Inst Food & Drug Control, Lanzhou 730000, Peoples R China;[3]Northwest Collaborat Innovat Ctr Tradit Chinese Me, Lanzhou 730000, Peoples R China;[4]TCM Coll Gansu Prov, Key Lab Chem & Qual, Lanzhou 730000, Peoples R China;[5]Gansu Prov Engn Lab TCM Standardizat Technol & Pop, Lanzhou 730000, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Lanzhou 730000, Peoples R China;[2]corresponding author), Lanzhou Inst Food & Drug Control, Lanzhou 730000, Peoples R China;[3]corresponding author), Northwest Collaborat Innovat Ctr Tradit Chinese Me, Lanzhou 730000, Peoples R China;[4]corresponding author), TCM Coll Gansu Prov, Key Lab Chem & Qual, Lanzhou 730000, Peoples R China;[5]corresponding author), Gansu Prov Engn Lab TCM Standardizat Technol & Pop, Lanzhou 730000, Peoples R China.|[10735]甘肃中医药大学;

年份：2022

卷号：2022

外文期刊名：JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY

收录：;Scopus(收录号：2-s2.0-85143975142);WOS:【SCI-EXPANDED(收录号:WOS:000896978400002)】；

基金：AcknowledgmentsThis work was supported by the National Natural Science Foundation of China (No. 82160457), Lanzhou Science and Technology Program (No. 2021-1-185), Medical Products Administration Program of Gansu Province (No. 2020GSMPA027), Natural Science Foundation of Gansu Province (No. 21JR7RA564), Program of Key Laboratory of Chemistry and Quality for Traditional Chinese Medicines College of Gansu Province (No. zzy-2022-07).

语种：英文

摘要：Shaoyao-Gancao Decoction (SGD) is a well-known classic traditional Chinese medicine (TCM) with antispasmodic, anti-inflammatory, and analgesic effects. This preparation has been widely used to treat spasticity diseases in the clinic. To date, the material basis of SGD remains unclear, and the spectrum-effect correlation of its antispasmodic activity has not been reported yet. In this study, high-performance liquid chromatography (HPLC) was used to establish the fingerprint and determine the multiple components of SGD. The common peaks of fingerprints were evaluated by the similarity with the chromatographic fingerprints of the TCM. Meanwhile, the multiple components were quantified and analysed using the heatmap and box size analysis. Furthermore, data on the antispasmodic effect were extracted through in vitro smooth muscle contraction assay. Grey relational analysis combined with partial least square regression was used to study the spectrum-effect correlation of SGD. Finally, the potential antispasmolytic components were validated using an isolated tissue experiment. The HPLC fingerprint was established, and 20 common peaks were identified. The similarities of 15 batches of SGD were all above 0.965. The HPLC method for simultaneous determination of the multiple components was accurate and reliable. The contents of albiflorin, paeoniflorin, liquiritin, and glycyrrhizic acid were higher than the other components in SGD. The heatmap and box size also showed that X3 (albiflorin), X4 (paeoniflorin), X5 (liquiritin), X11 (liquirtigenin), and X16 (glycyrrhizic acid) could be used as quality indicators in the further establishment of quality standards. The spectrum-effect correlation results indicated that X4, X11, and X16 were highly correlated with antispasmolytic activity. Verification tests showed that paeoniflorin (11.7-29.25 mu g/mL) and liquirtigenin (17.19-28.65 mu g/mL) could significantly reduce the maximum contractile (P < 0.01). These compounds exerted concentration-dependent spasmolytic effects with the inhibitory response for acetylcholine (Ach)-evoked contraction. Thus, SGD had a significant antispasmodic effect, which resulted from the synergistic activity of its multiple components. These findings can be used for the pharmacodynamics study of SGD and are of great significance for the determination of quality markers and quality control.