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中麻黄UPLC指纹图谱及其不同产区化学成分差异研究     被引量:3

UPLC Fingerprint and Chemical Components Difference Among Ephedra intermedia from Different Regions

文献类型:期刊文献

中文题名:中麻黄UPLC指纹图谱及其不同产区化学成分差异研究

英文题名:UPLC Fingerprint and Chemical Components Difference Among Ephedra intermedia from Different Regions

作者:马晓辉[1];卢有媛[1];张弦飞[2];黄得栋[1,3];朱田田[1];晋玲[1,3]

第一作者:马晓辉

机构:[1]甘肃中医药大学中(藏)药资源研究所,甘肃兰州730000;[2]中国科学院兰州化学物理研究所/中科院西北特色植物资源化学重点实验室和甘肃省天然药物重点实验室,甘肃兰州730000;[3]甘肃省高校中(藏)药化学质量研究省级重点实验室,甘肃兰州730000

第一机构:甘肃中医药大学科研实验中心(甘肃省中医药标准化技术委员会秘书处)

年份:2016

卷号:39

期号:10

起止页码:2217

中文期刊名:中药材

外文期刊名:Journal of Chinese Medicinal Materials

收录:CSTPCD;;Scopus;北大核心:【北大核心2014】;CSCD:【CSCD2015_2016】;PubMed;

基金:2014高校基本科研业务费专项课题(甘财教[2014]63号06课题);甘肃教育厅科研项目(1106B-07);科技基础性工作专项重点项目子课题(SB2007FY020)

语种:中文

中文关键词:中麻黄;UPLC;指纹图谱;化学成分

外文关键词:Ephedra intermedia Schrenk et C.A.Mey.; UPLC; Fingerprint; Chemical Components

摘要:目的:建立中麻黄的超高效液相色谱(UPLC)指纹图谱,为其质量控制提供科学依据,同时揭示不同产区化学成分之间的关系。方法:采用UPLC法建立中麻黄指纹图谱,利用内参比峰计算共有峰的相对峰面积。应用SPSS 19.0对不同产地中麻黄的化学成分进行系统聚类。结果:建立了中麻黄药材的UPLC指纹图谱,共标定了14个共有峰,化学成分的系统聚类结果显示,中麻黄化学成分西北产区聚为一类,山西产区聚为一类。结论:建立了稳定、可靠、重复性好的中麻黄指纹图谱,可用于其质量评价;不同产区中麻黄药材化学成分存在一定差异。
Objective: To establish the UPLC fingerprint of Ephedra intermedia,and to provide scientific basis for the quality control,meanwhile,to reveal the chemical components relationship of Ephedra intermedia in different regions. Methods: UPLC method was used to establish the fingerprint of Ephedra intermedia,and the relative peak area of common peaks was calculated based on the reference peak. The application of SPSS 19. 0 clustered the chemical components of Ephedra intermedia. Results: The UPLC fingerprint of Ephedra intermedia was established,and 14 common peaks were calibrated. The results of chemical composition systematic cluster analysis showed that the northeast region gathered for a class and Shanxi producing areas gathered for another class. Conclusion: The UPLC fingerprint of Ephedra intermedia is stable,reliable and reproducible,which could be used for quality evaluation.

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