文献类型：期刊文献

中文题名：连作及轮作对党参根际微生物多样性的影响

英文题名：Effects of Continuous Cropping and Crop Rotation on Rhizosphere Microbial Diversity of Codonopsis pilosula

作者：陈臻[1,2];曾翠云[3];张永合[1,2];万家秀[1,2]

第一作者：陈臻

机构：[1]兰州资源环境职业技术大学环境与化工学院,甘肃兰州730021;[2]兰州资源环境职业技术大学黄河流域生态环境产教融合研究院,甘肃兰州730021;[3]甘肃中医药大学药学院,甘肃兰州730000

第一机构：兰州资源环境职业技术大学环境与化工学院,甘肃兰州730021

年份：2023

卷号：46

期号：4

起止页码：823

中文期刊名：中药材

外文期刊名：Journal of Chinese Medicinal Materials

收录：CSTPCD;;北大核心:【北大核心2020】；PubMed;

基金：甘肃省自然科学基金项目(22JR5RA804);甘肃省高等学校创新基金项目(2020A-222);甘肃省高等学校青年博士基金项目(2022QB-103);2022年度兰州资源环境职业技术大学校级教学改革研究项目(JG22B07)。

语种：中文

中文关键词：党参;连作;轮作;土壤微生物;多样性指数

外文关键词：Codonopsis pilosula(Franch.)Nannf.;Continuous cropping;Crop rotation;Soil microorganism;Diversity indexes

摘要：目的:探明党参连作与轮作对根际土壤微生物群落组成的影响,为缓解党参连作障碍提供基础研究。方法:设置党参连作、马铃薯-党参轮作、玉米-党参轮作3个处理,测定党参的相关生长指标,并利用高通量测序技术,研究连作和轮作对党参根际土壤微生物多样性的影响。结果:与连作组相比,轮作组党参的根长、根粗、地下部分鲜重和干重以及根部产量均显著提高(P<0.05),轮作组根际土壤细菌群落OTU数目、丰富度指数Chao1和多样性指数Shannon也显著提高。连作组根际土壤细菌优势属种类比例均少于轮作组,但真菌特有OTU数量高于轮作组。党参连作与轮作土壤细菌在门分类水平上群落组成相对固定,变形菌门Proteobacteria、酸杆菌门Acidobacteria是党参轮作组根际土壤优势菌群,酸杆菌门、变形菌门、拟杆菌门Bacteroidetes为党参连作组根际土壤优势菌群。子囊菌门Ascomycota、担子菌门Basidiomycota为根际土壤真菌中的优势菌群;在属分类水平,连作组未分类的子囊菌及镰刀菌属的相对丰度高于轮作组。结论:与连作相比,轮作能够提高党参的生长指标和产量,增加根际细菌土壤微生物多样性,减少病原真菌种类,玉米-党参轮作模式相关指标优于马铃薯-党参轮作,能有效缓解连作障碍。
Objective:To investigate the effects of continuous cropping and crop rotation ofCodonopsis pilosulaon the composition of rhizosphere soil microbial community,so as to provide basic research for alleviating the obstacles of continuous cropping ofCodonopsis pilosula.Methods:Three treatments were set,including continuous cropping ofCodonopsis pilosula,crop rotation of potato andCodonopsis pilosula,crop rotation of corn andCodonopsis pilosula,the related growth indexes ofCodonopsis pilosulawere determined.The effects of continuous cropping and crop rotation on rhizosphere soil microbial diversity ofCodonopsis pilosulawere studied using high?throughput sequencing technology.Results:Compared with continuous cropping group,the root length,root diameter,fresh and dry weight of under?ground part and root yield ofCodonopsis pilosulawere significantly increased in crop rotation group(P<0.05),and the number of OTU,richness index Chao1 and diversity index Shannon of rhizosphere soil bacterial community were all significantly increased.The propor?tion of dominant genus species in rhizosphere soil of continuous cropping group was lower than that in crop rotation group,but the num?ber of fungus?specific OTU was higher than that in crop rotation group.At the phylum classification level,the community composition of soil bacteria inCodonopsis pilosulacontinuous cropping and crop rotation was relatively fixed.Proteobacteria and Acidobacteria were the dominant bacteria groups in the rhizosphere soil ofCodonopsis pilosulacrop rotation group,while Acidobacteria,Proteobacteria and Bacteroidetes were the dominant bacteria groups in the rhizosphere soil ofCodonopsis pilosulacontinuous cropping group.Ascomycota and Basidiomycota were the dominant microorganisms in rhizosphere soil fungi.At the genus classification level,the relative abundance of unclassified Ascomycota andFusariumin continuous cropping group was higher than that in crop rotation group.Conclusion:Com?pared with continuous cropping,crop rotation can improve the growth indexex and yield ofCodonopsis pilosula,increase the diversity of rhizosphere bacterial soil microorganism,and reduce the species of pathogenic fungi.Corn?Codonopsis pilosulacrop rotation is better than that of potato?Codonopsis pilosulacrop rotation,which can effectively alleviate continuous cropping obstacles.