文献类型：期刊文献

中文题名：扶正屏风合剂煎煮工艺与薄层色谱质控研究

英文题名：Study on decoction process and quality control of thin-layer chromatography of Fuzheng Pingfeng Mixture

作者：宋治荣[1];王耀鹏[1];杨锡仓[1];张兆芳[1]

第一作者：宋治荣

机构：[1]甘肃中医药大学附属医院炮制工程中心,甘肃兰州730020

第一机构：甘肃中医药大学第二附属医院

年份：2021

卷号：11

期号：15

起止页码：65

中文期刊名：中国医药科学

外文期刊名：China Medicine And Pharmacy

基金：甘肃省科技计划项目(20JR10RA337)。

语种：中文

中文关键词：扶正屏风合剂;提取工艺;正交试验;薄层色谱

外文关键词：Fuzheng Pingfeng Mixture;Extraction process;Orthogonal test;Thin-layerchromatography

摘要：目的建立扶正屏风合剂的煎煮工艺与薄层鉴别方法。方法以干浸膏率为客观评价指标,采用正交设计优选煎煮工艺;用3批中试样品进行薄层色谱研究。结果煎煮工艺为煮前先浸泡30 min,煎煮提取2次,每次加8倍量水,每次45 min;实验建立了方中黄芪、防风和连翘3味药的薄层鉴别方法。结论该煎煮提取工艺切实可行,薄层鉴别方法均专属性强、重现性好,可用于扶正屏风合剂的质量控制。
Objective To establish the decoction process and thin-layer identificati on method for Fuzheng Pingfeng Mixture.Methods Taking the dry extract yielding rate as an objective evaluation index,an orthogonal design was adopted to optimize the decoction process,and studies of thin-layer chromatography were conducted with three batches of pilot samples.Results The decoction process was soaking for 30 min before boiling and extracting twice,for 45 min each time with 8 times the amount of water added.The thinlayer identification method was established for three herbs in the formula,namely,milkvetch root(Huang Qi),divaricate saposhnikovia root(Fang Feng)and weeping forsythia capsule(Lian Qiao).Conclusion The decoction extraction process is practical and feasible,and the thin-layer identification method is highly specific and reproducible,which can be used for the quality co ntrol of Fuzheng Pingfeng Mixture.