文献类型：期刊文献

中文题名：葛根素对去卵巢大鼠认知功能及海马神经元超微结构的影响

英文题名：Effects of pueraria on the cognitive function and neuronal ultrastructure in hippocampus CA1 and CA3 of ovariectomized rats

作者：王雅莉[1];李长天[1];陈雁飞[2];程小丽[1];舍雅莉[1];胡树名[1]

第一作者：王雅莉

机构：[1]甘肃中医学院病理学教研室甘肃省中药药理与毒理学重点实验室,甘肃兰州730000;[2]兰州大学第一医院

第一机构：甘肃中医药大学基础医学院（敦煌医学研究所）|甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2013

卷号：33

期号：17

起止页码：4203

中文期刊名：中国老年学杂志

外文期刊名：Chinese Journal of Gerontology

收录：北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：甘肃省自然科学基金资助项目(No.0710RJZA011)

语种：中文

中文关键词：葛根素;去卵巢大鼠;海马神经元;凋亡

外文关键词：Puerarin ; Ovariectomized rats ; Hippocampal neuron ; Apoptosis

摘要：目的观察不同浓度葛根素对去卵巢大鼠认知功能及海马CA1区、CA3区神经元电镜下形态的影响。方法 SD大鼠72只,随机分为葛根素高、中、低剂量组、阳性对照组(倍美力组)、模型组、假手术组,每组12只,除假手术组外,均摘除大鼠卵巢造成去势模型。灌胃30 d后,用Morris水迷宫检测大鼠认知功能的变化,用透射电镜观察海马CA1区、CA3区神经元形态变化。结果在训练第6天,Morris水迷宫试验测试大鼠第一次经过平台的时间(潜伏期)与模型组比较,假手术组、阳性对照组和葛根素高剂量组有统计学意义(P<0.05);大鼠在120 s内经过平台的次数与模型组比较,假手术组、阳性对照组和葛根素高剂量组有统计学意义(P<0.01);与低剂量组比较,葛根素高剂量组有统计学意义(P<0.05)。透射电镜结果表明,葛根素在不同浓度(30～120 mg/kg)范围内,随着浓度的增大,抑制海马神经元细胞凋亡的作用越强,并有一定的剂量依赖关系。结论葛根素可以促进去卵巢大鼠学习、记忆功能的恢复,减轻因雌激素缺乏而引起的大鼠海马神经元凋亡作用。
Objective To observe the effect of puerarin on the cognitive function of ovariectomized rats and further study the effects of puerarin on neuron Scanning Electronic Microscopy（SEM） of ovariectomized rat＇s CA1 and CA3 region. Methods 72 of SPF female rats, weight（200 ± 20） g, were treated into ovariectomized rats and then randomly divided into：sham, model, positive control and puerarin treatment groups, 12 rats in each group. Rats in experimental groups were administered with saline and the corresponding drugs, respective-ly, by gavage for 30 days. At the end of the treatment, the cognitive function was tested by Morris water maze method. The morphology change of hippocampal neurons were observed by SEM. Results In the 6th day of training, compared with model group, there were signifi-cant differences in the exact time of the first passed through the platform and the times of passed through the platform in 120 seconds of sham, positive control and puerarin treatment groups（ P 〈 0. 05 ,P 〈 0. 01 ）. The results of SEM showed that there was obvious decrease of apoptosis phenomenon such as nuclei pyknosi and chromatin concentration in rats＇ hippocampal of puerarin treatment group. Conclusions Puerarin can improve the learning and memory functions of ovariectomized rats. Puerarin can decrease the apoptosis of rats＇ hippocampal neuron caused by lack of estrogen.