文献类型：期刊文献

中文题名：白藜芦醇对人骨髓间充质干细胞自发衰老的抑制作用

英文题名：Inhibition Effect of Resveratrol on Spontaneous Senescence of Human Bone Marrow-Derived Mesenchymal Stem Cells

作者：杨癑[1];何建新[2,3]

第一作者：杨玥

机构：[1]西北民族大学医学部;[2]甘肃中医药大学基础医学院;[3]甘肃中医药大学甘肃省中药药理与毒理学重点实验室,甘肃兰州730000

第一机构：西北民族大学医学部

年份：2024

卷号：32

期号：4

起止页码：1224

中文期刊名：中国实验血液学杂志

外文期刊名：Journal of Experimental Hematology

收录：CSTPCD;;Scopus;北大核心:【北大核心2023】；CSCD:【CSCD_E2023_2024】；PubMed;

基金：国家自然基金青年基金(81503377);甘肃省自然科学基金(1310RJZA083);甘肃中医药大学敦煌医学与转化重点实验室开放基金(DHYX18-10);西北民族大学引进人才科研项目(xbmuyjrc202235);甘肃省高校青年博士基金(2022QB-027)。

语种：中文

中文关键词：人骨髓间充质干细胞;白藜芦醇;自发衰老;hTERT;mTOR

外文关键词：human bone marrow-derived mesenchymal stem cell;resveratrol;spontaneous senescence;hTERT;mTOR

摘要：目的:观察白藜芦醇(RSV)对人骨髓间充质干细胞(MSC)的自发衰老是否具有抑制作用。方法:将MSC传至p13和p15代,建立MSC的自发衰老模型组;用5 nmol/L RSV分别处理p13和p15代MSC 48 h,建立RSV处理组。通过SA-β-Gal染色法检测细胞衰老,MTT法检测细胞增殖,RT-PCR检测细胞衰老相关端粒酶活性,Western blot检测与衰老相关的磷酸化mTOR的水平。结果:SA-β-Gal染色显示,RSV处理组MSC的衰老细胞数量明显少于模型组(p13代MSC,RSV组vs模型组,P<0.05;p15代MSC,RSV组vs模型组,P<0.01)。MTT结果显示,RSV处理组MSC的增殖能力高于模型组,其中p13代MSC处理组与模型组在72 h相比有显著差异(P<0.05)。RT-PCR结果显示,RSV处理组中MSC的hTERT mRNA表达量高于模型组,其中p13代MSC的RSV处理组与模型组相比有显著差异(P<0.05)。Western blot结果显示,RSV处理组中MSC的磷酸化(Ser2448)mTOR水平低于模型组,其中p13代MSC的处理组与模型组相比有显著差异(P<0.05)。结论:RSV可通过调节mTOR活性抑制人MSC的自发衰老。
Objective:To explore whether Resveratrol(RSV)can inhibit the spontaneous senescence of human bone marrow-derived mesenchymal stem cells(MSC).Methods:MSC were serially cultured to passage 13 and passage 15 to establish model groups exhibiting spontaneous senescence,respectively.MSC at passage 13 and passage 15 were treated with 5 nmol/L RSV for 48 h to establish the RSV-treated groups.SA-β-Gal staining was used to detect cell senescence.MTT assay was used to detect cell proliferation.RT-PCR method was used to detect senescence-associated telomerase activity.Western blot was used to detect the senescence-associated protein level of the phosphorylated-mTOR.Results:SA-β-Gal staining showed that the senescent cells of MSC in RSV-treated group was significantly less than those in the model group(RSV group compared with model group at passage 13,P<0.05;RSV group compared with model group at passage 15,P<0.01).The cell proliferation ability of MSC in RSV-treated group was significantly higher than those in model group,at 72 h in passage 13,there was significant difference between RSV-treated group and model group(P<0.05).RT-PCR results showed that the hTERT mRNA expression of MSC in RSV-treated group was higher than that in model group,which was significantly different between RSV-treated group and model group at passage 13(P<0.05).Western blot results showed that the phosphorylated(Ser2448)-mTOR level of MSC in RSV-treated group was lower than that in model group,which was significantly different between RSV-treated group and model group at passage 13(P<0.05).Conclusion:RSV can inhibit the spontaneous senescence of human MSC by mediating mTOR activity.