文献类型：期刊文献

中文题名：儿茶素对急性高原缺氧损伤的保护作用

英文题名：The protective effect of catechin on acute high-altitude hypoxic injury

作者：赵安鹏[1,2];马江红[1,3];王子晗[1,3];常熙雯[1,2];李文斌[1];王荣[1,2,3]

第一作者：赵安鹏

机构：[1]联勤保障部队第九四〇医院药剂科,甘肃兰州730050;[2]兰州大学药学院;[3]甘肃中医药大学药学院,甘肃兰州730000

第一机构：联勤保障部队第九四〇医院药剂科,甘肃兰州730050

年份：2023

卷号：39

期号：6

起止页码：1097

中文期刊名：中国药理学通报

外文期刊名：Chinese Pharmacological Bulletin

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2023_2024】；

基金：国家自然科学基金资助项目(No 82173738);中央高校基本科研业务费专项(No 3192020009);甘肃省青年科技基金计划项目(No 20JR10RA014)。

语种：中文

中文关键词：儿茶素;高原;抗缺氧;氧化应激;炎症;低压缺氧

外文关键词：catechin;plateau;anti-hypoxia;oxidative stress;inflammation;hypobaric hypoxia

摘要：目的研究儿茶素对大鼠急性高原缺氧损伤的保护作用。方法将大鼠按照6只/组随机分为对照组、高原低氧模型组、高原低氧+红景天胶囊组、高原低氧+儿茶素低剂量组、高原低氧+儿茶素中剂量组和高原低氧+儿茶素高剂量组。动物预防给药3 d后急进至海拔4010 m高原,继续给药5 d后,取腹主动脉血进行血气检测;取心肌、脑、肺组织,HE染色观察病理变化;测定心肌、脑、肺组织MDA含量、GSH含量、NO含量、SOD活力及血清IL-6、TNF-α含量。结果与对照组相比,高原低氧模型组大鼠血氧饱和度明显降低,心肌、脑和肺组织均出现不同程度的损伤,组织MDA含量升高,GSH含量降低,NO含量增加,SOD活力下降,血清炎症因子TNF-α、IL-6水平升高。预防给予儿茶素后,缺氧大鼠的血氧饱和度明显提高(P<0.05),HE染色结果显示心肌、脑、肺组织损伤得到不同程度的缓解,主要组织中MDA含量降低、GSH含量升高、SOD活力升高、NO含量下降,IL-6、TNF-α含量有所降低。结论儿茶素具有抗高原缺氧活性,对急进高原大鼠主要脏器缺氧损伤具有保护作用,其作用与缓解急性低氧暴露所致氧化应激和炎症有关。
Aim To study the protective effect of catechin on acute altitude injury in rats.Methods Rats were randomly divided into six groups:control group,altitude hypoxia model group,rhodiola capsule group,low-,middle-and high dose of catechin groups.After three days of preventive administration,animals were rushed to 4010 m altitude.After five days of continuous administration,abdominal aortic blood of rats was collected for blood gas detection.Cardiac,brain and lung tissues were collected for HE staining to observe the pathological changes.MDA content,GSH content,NO content,SOD activity of myocardial,brain and lung tissues were detected,so were IL-6 and TNF-αcontent in serum.Results Compared with the control group,blood oxygen saturation of rats of altitud hypoxia model group was significantly reduced,while myocardial,brain and lung tissues were damaged to different degrees.MDA and NO content increased,while GSH content and SOD activity decreased.The serum inflammatory factors TNF-αand IL-6 levels were elevated significantly.After catechin treatment,blood oxygen saturation of hypoxia rats significantly increased(P<0.05).HE staining results showed that myocardial,brain and lung tissue injury was alleviated to some extent.MDA,NO,IL-6 and TNF-αcontent were down-regulated,while GSH content and SOD activity were up-regulated respectively.Conclusions Catechin can resist high altitude hypoxia and protect the main organs from hypoxia injury in rats acute exposed to altitude,which is related to alleviating oxidative stress and inflammation caused by acute hypoxia exposure.