文献类型：期刊文献

中文题名：黄芪多糖对肺癌微环境中BMSCs增殖及TAFs分化的影响

英文题名：Effects of Astragalus polysaccharides on proliferation and expression of TAFs related molecule of bone marrow mesenchymal stem cells in the lung cancer microenvironment

作者：武有明[1];张齐[1];刘永琦[1,2];何建新[1];伍志伟[1,2];高卓越[1];苏韫[1];骆亚莉[1];张利英[1];周妮娜[1]

第一作者：武有明

机构：[1]甘肃中医学院,甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室,兰州730000;[2]敦煌医学与转化省部共建教育部重点实验室,兰州730000

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2015

卷号：31

期号：6

起止页码：76

中文期刊名：中药药理与临床

外文期刊名：Pharmacology and Clinics of Chinese Materia Medica

收录：北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：甘肃省杰出青年基金项目,NO:1308RJDA008;国家自然科学基金项目,NO:81360588

语种：中文

中文关键词：黄芪多糖;骨髓间充质干细胞;肿瘤微环境;增殖;分化;肿瘤相关成纤维细胞

外文关键词：Astragalus polysaccharides （ 黄芪多糖 ） ; bone marrow mesenchymal stem cells; tumor microenvironment; proliferation; differ-entiation; tumor associated fibroblasts

摘要：目的:探讨黄芪多糖(APS)对肺癌微环境中骨髓间充质干细胞(bone mesenchymal stem cells,BMSCs)增殖、分化及肿瘤相关成纤维细胞(Tumor-associated Fibroblasts,TAFs)相关分子表达的影响。方法:采用CCK-8法筛选黄芪多糖作用于肺癌细胞Lewis(Lewis Lung Cancer,LLC)、BMSCs的最佳药物浓度;利用Transwell小室建立BMSCs与LLC细胞的共培养体系,并以黄芪多糖最佳药物浓度干预该体系;通过CCK-8法、流式细胞术及Western blot分别检测黄芪多糖对共培养体系中BMSCs细胞增殖、周期及TAFs标记分子α-SMA、FAP蛋白的表达变化。结果:50 mg/L黄芪多糖可促进BMSCs细胞增殖,同时对LLC细胞有明显抑制作用;与正常对照组相比,共培养组细胞生长速度加快,G0/G1期比例降低,S期比例升高,且α-SMA、FAP蛋白表达显著增加;与共培养组相比,黄芪多糖组(50mg/L)生长速度减慢,G0/G1期比例升高,S期比例降低,且α-SMA、FAP蛋白表达显著降低。结论:肺癌微环境中BMSCs细胞形态、增殖特性发生异常改变,具有向肿瘤相关成纤维细胞分化性,且黄芪多糖可抑制肺癌微环境中BMSCs的异常改变。
Objective： To investigate effects of Astragalus polysaccharides（APS） on proliferation and expression of TAFs related molecule of bone marrow mesenchymal stem cells（BMSCs） in the lung cancer microenvironment. Methods： The optimal concentration of APS on the effect of lewis lung cancer （LLC） and BMSCs was selected by CCK-8; after establishing the co-culture system of LLC and BMSCs by using Transwell Chambers,we intervened it by the effective concentration of APS; the morphology of cells was observed by phase-contrast microscopy, the proliferation capability of cells was tested by CCK-8, the cell cycle was tested by flow cytometry, the protein expression of biomarkers of TAFs-α-SMA and FAP of each group BMSCs was detected by Western blot. Results： Compared with the control group, 50 ug/ml APS group promoted BMSCs cells proliferation while had an obvious inhibitory effect on LLC cells proliferation; Compared with the control group, the growth rate of co-culture group increased significantly, the ratio of cells in G0/G1 phase of co-culture group was reduced with an increase in S phase; Compared with co-culture group, the proportion of ceils in G0/G1 phase of APS group was increased, and the proportion of cells in S phase was significant decreased ; In the results of Western blot, the protein expression of ct-SMA and FAP in co-culture group raised obvi- ously compared with that in control group, Compared with co-culture group, the expression of c^-SMA and FAP protein decreased in the group of APS group. Conclusion： BMSCs in lewis lung cancer microenvironment changes abnormally in cell morphology and proliferation character- istics and has a trend to the tumor fibroblast differentiation, and APS could inhibit the abnormal changes.