文献类型：期刊文献

中文题名：甘南栽培唐古特大黄抗炎作用及机制分析

英文题名：Analysis of Anti-inflammatory Effect and Mechanism of Rheum tanguticum Cultivated in South of Gansu

作者：王志旺[1];郭玫[1];马丹[1];妥海燕[1];王瑞琼[1]

第一作者：王志旺

机构：[1]甘肃中医药大学药学院

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2016

卷号：22

期号：1

起止页码：158

中文期刊名：中国实验方剂学杂志

外文期刊名：Chinese Journal of Experimental Traditional Medical Formulae

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD_E2015_2016】；

基金：甘南州科技开发交流中心基金项目(BH2012-H01)

语种：中文

中文关键词：甘南栽培唐古特大黄;耳肿胀;足肿胀;白三烯B4;对比研究

外文关键词：Rheum tanguticum cultivated in Gannan state; auricular swelling; foot swelling; leukotriene B4; comparative study

摘要：目的:对比研究甘南栽培唐古特大黄抗炎作用及机制。方法:分别选取SPF级KM小鼠及SPF级雄性Wistar大鼠,通过二甲苯致小鼠耳肿胀法、甲醛致小鼠足肿胀法及角叉菜胶致大鼠足肿胀法来对比考察甘南栽培唐古特大黄高、低剂量(小鼠给药剂量4.5,1.5 g·kg-1,大鼠给药剂量3.0,1.0 g·kg-1),西宁栽培唐古特大黄高、低剂量(小鼠给药剂量4.5,1.5 g·kg-1,大鼠给药剂量3.0,1.0 g·kg-1)与礼县栽培掌叶大黄高、低剂量(小鼠给药剂量4.5,1.5 g·kg-1,大鼠给药剂量3.0,1.0 g·kg-1)的抗炎作用;复制小鼠气囊滑膜炎,通过测定炎性渗出物中总蛋白及白三烯B4(LTB4)的含量对比考察大黄高、低剂量的抗炎机制,实验中另外设正常组。结果:与正常组比较,1.5,4.5 g·kg-1甘南栽培唐古特大黄、西宁栽培唐古特大黄与礼县栽培掌叶大黄可明显缓解小鼠耳廓肿胀度与小鼠和大鼠(1.0,3.0 g·kg-1)足肿胀度(P<0.05,P<0.01);与模型组比较,1.5,4.5 g·kg-1甘南栽培唐古特大黄、西宁栽培唐古特大黄与礼县栽培掌叶大黄可明显降低气囊滑膜炎渗出物中总蛋白与LTB4的含量,而甘南栽培唐古特大黄、西宁栽培唐古特大黄抑制炎性渗出物中总蛋白与LTB4的作用明显优于礼县栽培掌叶大黄(P<0.05,P<0.01)。结论:3种人工栽培大黄有明显的抗炎作用;唐古特大黄的部分抗炎作用优于掌叶大黄,而甘南栽培唐古特大黄与西宁栽培唐古特大黄的抗炎作用无明显差异;抑制LTB4生成是3种大黄抗炎机制之一。
Objective： To conduct a comparative study on anti-inflammatory effect and its mechanism of Rheum tanguticum cultivated in Ganan state（ RT-GN）. Method： The SPF KM mice and SPF male Wistar rats were selected,and with the methods of xylene-induced mice ear swelling,formaldehyde-induced mice foot swelling and carrageenan-induced rats foot swelling,they were compared with high and low doses R. tanguticum cultivated in south of Gansu（ 4. 5,1. 5 g·kg^-1for mice,and 3. 0,1. 0 g·kg^-1for rats）,high and low doses R. tanguticum cultivated in Xining（ 4. 5,1. 5 g·kg^-1for mice,and 3. 0,1. 0 g·kg^-1for rats） and high and low doses R.palmatum cultivated in Lixian County（ 4. 5,1. 5 g·kg^-1for mice,and 3. 0,1. 0 g·kg^-1for rats） for antiinflammatory effects. Mice airbag synovitis was replicated,and the determination of total protein and leukotriene B4（ LTB4） in the inflammatory exudate was done to comparatively investigate the anti-inflammatory mechanism of high and low dose rhubarb. Normal group was also set in the experiment. Result： Compared with the normal group,R. tanguticum cultivated in south of Gansu（ RT-GN）,R. tanguticum cultivated in Xining（ RT-XN）,and R. palmatum cultivated in Lixian（ RP-LX） could remit degree of auricular swelling in mice and foot swelling in mice and rats（ 1. 0,3. 0 g·kg^-1）（ P〈0. 05,P〈0. 01）. Compared with the model group,1. 5,4. 5 g·kg^-1RTGN,RT-XN,and RP-LX could significantly reduce total protein and LTB4 in airbag synovitis exudates,and the effects of RT-GN and RT-XN on total protein and LTB4 in inflammatory exudates were superior to that of RP-LX（ P〈0. 05,P〈0. 01）. Conclusion： Three kinds of cultivated Rhubarb have obvious anti-inflammatory effect,part anti-inflammatory effect of R. Tanguticum was superior to that of R. palmatum,but there was no significant difference in anti-inflammatory effect between RT-GN and RT-XN,and one of their mechanisms is to inhibit the production of LTB4.