文献类型：期刊文献

英文题名：Carbon ion radiotherapy boosts anti-tumour immune responses by inhibiting myeloid-derived suppressor cells in melanoma-bearing mice

作者：Zhou, Heng[1,2,3];Yang, Pengfei[1,2,3];Li, Haining[4];Zhang, Liying[5];Li, Jin[1,2,6];Zhang, Tianyi[1,2,3];Sheng, Chengyan[6];Wang, Jufang[1,2,3]

第一作者：Zhou, Heng

通信作者：Zhou, H[1];Wang, JF[1];Zhou, H[2];Wang, JF[2];Zhou, H[3];Wang, JF[3]

机构：[1]Chinese Acad Sci, Inst Modern Phys, Key Lab Space Radiobiol Gansu Prov, Lanzhou, Peoples R China;[2]Chinese Acad Sci, Inst Modern Phys, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou, Peoples R China;[3]Univ Chinese Acad Sci, Beijing, Peoples R China;[4]Gansu Prov Canc Hosp, Gansu Prov Acad Inst Med Sci, Lanzhou, Peoples R China;[5]Gansu Univ Chinese Med, Lanzhou, Peoples R China;[6]Lanzhou Univ, Sch Nucl Sci & Technol, Lanzhou, Peoples R China

第一机构：Chinese Acad Sci, Inst Modern Phys, Key Lab Space Radiobiol Gansu Prov, Lanzhou, Peoples R China

通信机构：[1]corresponding author), Chinese Acad Sci, Inst Modern Phys, Key Lab Space Radiobiol Gansu Prov, Lanzhou, Peoples R China;[2]corresponding author), Chinese Acad Sci, Inst Modern Phys, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou, Peoples R China;[3]corresponding author), Univ Chinese Acad Sci, Beijing, Peoples R China.

年份：2021

卷号：7

期号：1

外文期刊名：CELL DEATH DISCOVERY

收录：;Scopus(收录号：2-s2.0-85118505428);WOS:【SCI-EXPANDED(收录号:WOS:000714376400003)】；

基金：This work was supported by the Hundred-Talent Program of the Chinese Academy of Sciences (No. 29Y763050, Heng Zhou), the National Nature Science Foundation of China (No. 11905264, Heng Zhou), the Science and Technology Research Project of Gansu Province (Nos. 17JR5RA307 and 145RTSA012, Jufang Wang).

语种：英文

摘要：Numerous studies have shown that carbon ion radiotherapy (CIRT) induces anti-cancer immune responses in melanoma patients, yet the mechanism remains elusive. The abundance of myeloid-derived suppressor cells (MDSC) in the tumour microenvironment is associated with therapeutic efficacy and disease outcome. This study analysed the changes in the immune contexture in response to the carbon ion treatment. The murine melanoma B16, MelanA, and S91 tumour models were established in syngeneic immunocompetent mice. Then, the tumours were irradiated with carbon ion beams, and flow cytometry was utilised to observe the immune contexture changes in the bone marrow, peripheral blood, spleen, and tumours. The immune infiltrates in the tumour tissues were further assessed using haematoxylin/eosin staining and immunohistochemistry. The immunoblot detected the expression of proteins associated with the JAK/STAT signalling pathway. The secretion of immune-related cytokines was examined using ELISA. Compared to conventional radiotherapy, particle beams have distinct advantages in cancer therapy. Here, the use of carbon ion beams (5 GyE) for melanoma-bearing mice was found to reduce the population of MDSC in the bone marrow, peripheral blood, and spleen of the animals via a JAK2/STAT3-dependent mechanism. The percentage of CD3(+), CD4(+), CD8(+) T cells, macrophages, and natural killer cells increased after radiation, resulting in reduced tumour growth and prolonged overall survival in the three different mouse models of melanoma. This study, therefore, substantiated that CIRT boosts anti-tumour immune responses via the inhibition of MDSC.