文献类型：期刊文献

中文题名：加味当归贝母苦参丸对胃癌荷瘤鼠肿瘤组织VEGFA、MMP13和TGF-β表达的影响

英文题名：Effects of Modified Danggui Beimu Kushen Pill on the Expressions of VEGFA,MMP13 and TGF-β in Tumor Tissues of MFC Gastric Cancer Bearing Mice

作者：樊晓明[1];翟宇[2,3];安霞[4];吴红彦[3];李海龙[2,3]

第一作者：樊晓明

机构：[1]甘肃省定西市中医院药剂科,甘肃定西743000;[2]甘肃中医药大学临床医学院临检基础教研室,甘肃兰州730000;[3]甘肃省中医方药挖掘与创新转化重点实验室甘肃省中药新产品创制工程实验室甘肃省中药药理与毒理学重点实验室,甘肃兰州730000;[4]甘肃中医药大学附属医院,甘肃兰州730000

第一机构：甘肃省定西市中医院药剂科,甘肃定西743000

年份：2018

卷号：46

期号：4

起止页码：31

中文期刊名：中医药学报

外文期刊名：Acta Chinese Medicine and Pharmacology

收录：CSTPCD

基金：甘肃省中药药理与毒理学重点实验室开放基金项目(ZDSYS-KJ-2015-010)

语种：中文

中文关键词：加味当归贝母苦参丸;胃癌;MMP13;TGF-β

外文关键词：Modified Danggui Beimu Kushen Pill;Gastric cancer;MMP13;TGF-β

摘要：目的:研究当归贝母苦参丸加味方对MFC胃癌荷瘤小鼠瘤组织VEGFA、MMP13和TGF-β表达的影响。方法:建立MFC胃癌荷瘤小鼠模型进行体内抗肿瘤试验,将筛选合格的48只MFC胃癌荷瘤小鼠随机分为模型组、顺铂阳性对照组、加味当归贝母苦参丸高、低剂量组、顺铂联合加味当归贝母苦参丸高、低剂量组,连续灌胃给药14 d,治疗结束后,处死大鼠,RT-q PCR和免疫组织化学技术分别检测肿瘤组织中VEGFA、MMP13和TGF-β的表达。结果:与模型组相比,各用药组中VEGFA、MMP13和TGF-βmRNA和蛋白的表达量均降低;VEGFA、MMP13和TGF-β在联合组中蛋白着色变浅,呈弱阳性表达,联合用药组较顺铂组、当高、低组蛋白表达量降低。结论:当归贝母苦参丸加味方可以下调荷瘤小鼠肿瘤组织VEGFA、MMP13和TGF-β的表达,进而发挥抗肿瘤作用。
Objective： To study the effects of Modified Danggui Beimu Kushen Pill on the expressions of VEGFA,MMP13 and TGF-β in tumor tissues of MFC gastric cancer bearing mice. Methods： MFC gastric cancer bearing mice were employed to perform anti-tumor experiment in vivo. A total of 48 eligible mice were randomly divided into the model group,DDP group,low-dose group of Modified Danggui Beimu Kushen Pill,high-dose group of Modified Danggui Beimu Kushen Pill,DDP combined with low-dose group,and DDP combined with high-dose group. The treatment course was 14 continuous days. After the last treatment,all mice were killed to obtain complete tumor tissue for further detection of VEGFA,MMP13 and TGF-β with the method of RT-q PCR and immunohistochemistry. Results： RT-q PCR detection showed that the expressions of VEGFA,MMP13 and TGF-β mRNA in each medication group were decreased compared to those in the model group（ P〈 0. 05）. Immunohistochemical detection showed that the expressions of VEGFA,MMP13 and TGF-β were less colored and with weakly positive expression in the combined group,compared to DDP group and Chinese medication groups. Conclusion： Modified Danggui Beimu Kushen Pill can down-regulate the expressions of VEGFA,MMP13 and TGF-β of tumor tissue in MFC gastric cancer bearing mice to play anti-tumor role.