文献类型：期刊文献

中文题名：基于转录组学的归芪益元膏对^(12)C^(6+)束辐射损伤大鼠肺肾旁效应的作用机制

英文题名：Effects of lung and kidney bystander effect of Guiqi Yiyuan Ointment on rat with radiation damage by ^(12)C^(6+) beam based on transcriptome

作者：梁建庆[1,2,3];李金田[1,2,4];李娟[3];曾启宇[4];杜雪洋[4];胡蓉[4];刘凤艳[5];安耀荣[3];王军[5];孙雪[3];李长天[3];王虎平[3];蔺兴遥[1]

第一作者：梁建庆

机构：[1]甘肃中医药大学敦煌医学与转化省部共建教育部重点实验室;[2]甘肃中医药大学博士后流动站;[3]甘肃中医药大学基础医学院;[4]甘肃中医药大学中医临床学院;[5]甘肃中医药大学附属医院

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2019

卷号：0

期号：5

起止页码：2130

中文期刊名：中华中医药杂志

外文期刊名：China Journal of Traditional Chinese Medicine and Pharmacy

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD_E2019_2020】；

基金：国家自然科学基金地区项目(No.81460695;No.81760836);中国博士后科学基金第60批面上资助项目(No.2016M602887)~~

语种：中文

中文关键词：转录组学;归芪益元膏;辐射损伤;旁效应机制

外文关键词：Transcriptome;Guiqi Yiyuan Ointment;Radiation injury;Bystander effect mechanism

摘要：目的:探讨归芪益元膏对^(12)C^(6+)束辐射损伤大鼠旁效应的防护机制。方法:采用^(12)C^(6+)束照射大鼠右侧肺部构建辐射损伤动物模型,分层随机分为单纯辐射组、归芪益元膏组,同时设立正常对照组,取大鼠的左肺、左肾组织以提取总RNA,IlluminaHiSeq平台测序,检测各组大鼠所有基因转录本的差异表达,筛选出与旁效应保护相关的基因及代谢途径。结果:给予归芪益元膏能改善^(12)C^(6+)束辐射损伤大鼠旁效应,转录数据分析显示其作用机制主要涉及肿瘤坏死因子、细胞外基质、细胞-细胞黏附连接、钙离子、白细胞介素-1、4、8等蛋白基因表达上调/下调。结论:归芪益元膏对^(12)C^(6+)束辐射损伤大鼠旁效应具有一定的防护作用,其机制可能与抑制电离辐射响应及癌症信号转导通路有关。
Objective:To investigate the protective mechanism of Guiqi Yiyuan Ointment on the bystander effect of 12C6+beam radiation injury in rats.Methods:12C6+was used to irradiate the right lung of rats to construct an animal model of radiation injury.Rats were stratified and randomly divided into simple radiation group and Guiqi Yiyuan Ointment group.At the same time,Normal control group was also set up.Left lung and left kidney tissues of rats were taken to extract total RNA,and sequenced them on Illumina HiSeq platform.The differential expression of all transcripts in each group was detected.Screening for genes and metabolic pathways related to bystander effect protection.Results:Guiqi Yiyuan Ointment could improve the bystander effect of 12C6+beam radiation injury in rats.Transcriptional data analysis showed that the mechanism was mainly related to the up regulation/down regulation of protein and gene expressions,such as tumor necrosis factor,extracellular matrix,cell-cell adhesion junction,calcium ion,interleukin-1,4,8.Conclusion:Guiqi Yiyuan Ointment has a protective effect on the bystander effects of 12C6+beam radiation injury in rats.The mechanism may be related to the inhibition of ionizing radiation response and cancer signaling transduction pathway.