文献类型：期刊文献

中文题名：HPLC-DAD鉴别皂角刺中掺入野皂角刺的方法研究

英文题名：Identification of Gleditsia heterophylla Bunge. Adulterated in Gleditsia sinensis Lam. by HPLC-DAD

作者：刘小燕[1];夏巧红[1];宋平顺[1];张义福[2];贺军权[1];倪琳[1]

第一作者：刘小燕

机构：[1]甘肃省药品检验研究院国家中药材及饮片质量控制重点实验室,甘肃兰州730070;[2]甘肃中医药大学药学院,甘肃兰州730030

第一机构：甘肃省药品检验研究院国家中药材及饮片质量控制重点实验室,甘肃兰州730070

年份：2021

卷号：35

期号：4

起止页码：15

中文期刊名：现代中药研究与实践

外文期刊名：Research and Practice on Chinese Medicines

收录：CSTPCD

基金：国家重点研发计划“中医药现代化研究”重点专项(2019YFC1711500);甘肃省科技重大专项(17ZD2FA009)。

语种：中文

中文关键词：皂角刺;野皂角刺;阿魏酸;特有峰;HPLC-DAD

外文关键词：Gleditsia sinensis Lam.;Gleditsia heterophylla Bunge.;ferulic acid;peak S;HPLC-DAD

摘要：目的建立皂角刺中非法掺入野皂角刺的高效液相色谱鉴别法。方法取不同来源产地的皂角刺与野皂角刺,采用HPLC-DAD法测定其提取物化学组分,利用阿魏酸对照品作为内标物,以色谱相对保留时间及紫外光谱进行联合定性,检测HPLC图谱中野皂角刺特有峰,建立皂角刺中掺入野皂角刺检验方法,并进行方法学考察。结果伪品野皂角刺中检出一特有峰,其相对于阿魏酸的保留时间为1.43,正品皂角刺药材及饮片中均未检出该组份。方法精密度、重复性、稳定性、耐用性均良好。结论建立的方法操作简便,专属性强,可用于快速筛查皂角刺中掺入野皂角刺的情况,为皂角刺的质量控制提供实验依据。
Objective To establish a high performance liquid chromatography(HPLC) method of Gleditsia heterophylla Bunge. illegal adulteration in Gleditsia sinensis Lam.. Methods In order to find the peak S of Gleditsia heterophylla Bunge., Gleditsia sinensis Lam. and Gleditsia heterophylla Bunge. samples from different origins and sources are determined of chemical components with HPLC-DAD, by locating the peak with the UV spectra and the retention time relative to ferulic acid reference substance as an internal standard. Therefore, the adulteration detection method is established, and the methodology is investigated. Results The peak S is detected in all the Gleditsia heterophylla Bunge. products of which retention time relative to the ferulic acid peak is 1.43, and meanwhile the peak S is not detected in all the Gleditsia sinensis Lam. herbs and pieces. The precision, repeatability, stability and durability of the method are all good. Conclusion The adulteration detection method is simple and specific, and can be used for rapid discrimination of adulterated Gleditsia heterophylla Bunge. in Gleditsia sinensis Lam., which provide reference and basis for the quality control of Gleditsia sinensis Lam..