文献类型：期刊文献

中文题名：当归红芪超滤物通过调控P53表达对H9C2细胞凋亡的干预效应研究

英文题名：Study on the intervention effect of Radix Angelica Sinensis and Radix Hedysari ultrafiltration on H_(9)C_(2) cell apoptosis by regulating the expression of P53

作者：韩金晏[1];蒋虎刚[1,2];王新强[1];赵晓彬[1];刘凯[1];李应东[1,2];赵信科[1,2,3]

第一作者：韩金晏

机构：[1]甘肃中医药大学中西医结合学院,兰州730000;[2]甘肃中医药大学附属医院心血管临床医学中心,兰州730000;[3]甘肃省中医药防治慢性疾病重点实验室,兰州730000

第一机构：甘肃中医药大学中西医结合学院

年份：2023

卷号：38

期号：4

起止页码：1543

中文期刊名：中华中医药杂志

外文期刊名：China Journal of Traditional Chinese Medicine and Pharmacy

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD2023_2024】；

基金：甘肃省高等学校青年博士基金项目(No.2021QB-080);国家自然科学基金项目(No.81860786);甘肃省教育厅科技创新项目(No.2021jyjbgs-03);2021年度甘肃中医药大学科学研究与创新基金项目(No.2021KCYB-5);2022年陇原青年创新创业人才项目。

语种：中文

中文关键词：当归红芪超滤物;放射性心肌损伤;心肌细胞;细胞凋亡;P53;机制

外文关键词：Radix Angelica Sinensis and Radix Hedysari ultrafiltration;Radiation-induced myocardial injury;Cardiomyocyte;Apoptosis;P53;Mechanism

摘要：目的:阐明P53高表达能否介导H_(9)C_(2)细胞凋亡及当归红芪超滤物(RAS-RH)能否通过调控P53表达干预H_(9)C_(2)细胞凋亡。方法:将H_(9)C_(2)细胞随机分为正常组,阴性组,模型组和RAS-RH低、中、高剂量组。采用CCK-8法检测各组细胞增殖率,EDU染色检测各组H_(9)C_(2)心肌细胞的凋亡,免疫荧光检测Bax、Bcl-2的表达,鬼笔环肽染色观察细胞骨架结构,Western Blot检测P53、Caspase-3、Caspase-8蛋白表达。结果:与阴性组比较,模型组H_(9)C_(2)细胞存活率显著降低(P<0.05);EDU标记的阳性细胞明显减少;Bax表达上调,Bcl-2表达下调;H_(9)C_(2)细胞微丝微管变短,排列紊乱;P53、Caspase-3、Caspase-8蛋白表达均显著上调(P<0.05)。与模型组比较,RAS-RH低、中、高剂量组H_(9)C_(2)细胞存活率显著增加(P<0.05);EDU标记的阳性细胞增多;Bax表达下调,Bcl-2表达上调;H_(9)C_(2)细胞微丝微管结构排列规则,扭曲、断裂减少;P53、Caspase-3、Caspase-8蛋白表达均显著降低(P<0.05)。结论:RAS-RH可通过下调P53表达抑制X线辐射诱导的H_(9)C_(2)细胞凋亡。
Objective:To clarify whether the high expression of P53 can mediate the apoptosis of H_(9)C_(2) cells and whether the ultrafiltration of Radix Angelica Sinensis and Radix Hedysari(RAS-RH)can interfere with the apoptosis of H_(9)C_(2) cells by regulating the expression of P53.Methods:H_(9)C_(2) cells were randomly divided into normal group,negative group,model group,and low,medium and high dose groups of RAS-RH.The proliferation rate of cells in each group was detected by CCK-8 method,the apoptosis of H_(9)C_(2) cardiomyocytes in each group was detected by EDU staining,the expressions of Bax and Bcl-2 were detected by immunofluorescence,and the cytoskeleton structure was observed by phalloidin staining.Western Blot was used to detect the expression of P53,Caspase-3 and Caspase-8 proteins.Results:Compared with the negative group,the survival rate of H_(9)C_(2) cells in the model group was significantly decreased(P<0.05),EDU-labeled positive cells were decreased,the expression of Bax was up-regulated and Bcl-2 down-regulated,H_(9)C_(2)cells were microfilament microtubules shortened and disordered,the protein expressions of P53,Caspase-3 and Caspase-8 were all up-regulated(P<0.05).Compared with the model group,the survival rate of H,C,cells in the RAS-RH low,medium and high dose groups was significantly increased(P<0.05);EDU marked positive cells were increased,Bax expression was down-regulated and Bcl-2 expression was up-regulated H_(9)C_(2) cells microfilaments and microtubules were arranged regularly,with fewer twists and breaks;the protein expressions of P53,Caspase-3 and Caspase-8 were all decreased(P<0.05).Conclusion:RAS-RH can significantly inhibit the apoptosis of H,C,cells induced by X-ray radiation by down-regulating the expressionof P53.