文献类型：期刊文献

中文题名：整合多组学方法揭示D-半乳糖致衰老模型小鼠的脑-肠互动

英文题名：Integrated Multi-omics Approach Reveals the Interaction of Brain-gut in D-galactose-induced Aging Model Mice

作者：王晶[1];韩乐[1];康甲超[1,2];蒙洁[1,2];陈冬梅[1,3];吴萍民[4];田一虹[1];段永强[5]

第一作者：王晶

机构：[1]甘肃中医药大学公共卫生学院,兰州730000;[2]甘肃省妇幼保健院临床检验中心,兰州730000;[3]大庆油田总院检验诊断科,黑龙江大庆163000;[4]甘肃中医药大学教学实验中心,兰州730000;[5]宁夏医科大学中医学院,银川750004

第一机构：甘肃中医药大学公共卫生学院

年份：2023

卷号：39

期号：9

起止页码：1332

中文期刊名：中国生物化学与分子生物学报

外文期刊名：Chinese Journal of Biochemistry and Molecular Biology

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD_E2023_2024】；PubMed;

基金：国家自然科学基金项目(No.81760835,No.82060829)资助。

语种：中文

中文关键词：代谢组学;16S rDNA;D-半乳糖;衰老;脑-肠互动

外文关键词：metabolomics;16S rDNA;D-galactose;aging;interaction of brain-gut

摘要：衰老过程中会有多个器官在生理和病理上相互联系,而大脑在这一过程中发挥核心作用。大脑与肠道之间存在一种称为“脑-肠互动”的直接双向通信,这对研究衰老具有重要意义。本研究旨在探索脑-肠互动背景下机体衰老的机制。小鼠的一般体征结果显示,衰老小鼠的运动量减少,体重及摄食量也明显下降(P<0.001,P<0.05)。衰老小鼠的胸腺指数明显低于正常组(P<0.05),且胸腺病理结果显示衰老小鼠胸腺组织皮质变薄,髓皮质边界模糊,细胞排列较松散。代谢组学分析显示,粪便中有317个差异代谢物,海马中有100个差异代谢物。微生物组学结果显示,拟杆菌门和厚壁菌门是肠道菌群的优势菌门,衰老后拟杆菌门水平呈上调趋势,厚壁菌门水平呈下调趋势。KEGG通路结果显示,有26条代谢途径与衰老研究相关,其中对脑肠轴有重要意义的分别为半乳糖代谢、ABC转运体和嘌呤代谢。三组Spearman相关性分析结果显示,涉及的代谢物类型主要为脂质及类脂分子和有机酸及衍生物,涉及的肠道菌群主要为拟杆菌门和厚壁菌门。总之,本研究证明,衰老小鼠大脑和肠道之间存在的协同变化与衰老机制有关,这为发现机体衰老过程的机制提供了新研究思路。
Multiple organs are physiologically and pathologically interconnected during aging,and the brain plays a central role in this process.There is a direct two-way communication between the brain and the gut called“brain-gut interaction”,which is of great significance for the study of aging,and the mo-lecular mechanism remains to be further studied.The aim of this study is to explore the mechanism of ag-ing in the context of brain-gut interaction.The results of general physical signs of mice showed that the a-mount of exercise decreased,body weight and food intake decreased significantly in aged mice(P<0.001,P<0.05).The thymus index of aged mice was significantly lower than that of normal mice(P<0.05),and the thymic pathological results showed that the thymic cortex of aging mice was thinner,the boundary between medulla and cortex was blurred,and the cells were loosely arranged.Metabolomics a-nalysis revealed 317 differential metabolites in feces and 100 differential metabolites in hippocampus.The results of microbiome showed that Bacteroidetes and Firmicutes were the dominant phyla of gut microbiota.Bacteroidetes showed an upward trend and Firmicutes showed a downward trend after aging.KEGG path-way results showed that 26 metabolic pathways were related to the study of aging,among which galactose metabolism,ABC transporter and purine metabolism were of great significance for the brain-gut interac-tion.The results of Spearman correlation analysis of the three groups showed that the types of metabolites involved were mainly lipids and lipid-like molecules and organic acids and derivatives,and the gut micro-biota involved were mainly Bacteroidetes and Firmicutes.In conclusion,the present study demonstrated that the synergistic changes between brain and gut in aging mice were related to the mechanism of aging,which provided new insights into the mechanism of aging process.