文献类型：期刊文献

英文题名：Highly selective peptide-based fluorescent turn-on probe with large Stokes shift for the detection of Cd(II) and its applications in bio-imaging and smartphone-assisted recognition

作者：Deng, Weiliang[1];Liu, Jiayu[1];Zhao, Shanling[1];Liu, Zhuo[1];Wang, Yiwen[1];Wang, Peng[1];An, Yong[2];Wu, Jiang[3]

第一作者：Deng, Weiliang

通信作者：Wang, P[1];An, Y[2];Wu, J[3]

机构：[1]China West Normal Univ, Coll Chem & Chem Engn, Chem Synth & Pollut Control Key Lab Sichuan Prov, Shida Rd 1, Nanchong 637009, Peoples R China;[2]Gansu Univ Chinese Med, Sch Clin Med 1, Lanzhou 730030, Gansu, Peoples R China;[3]Qinghai Nationalities Univ, Coll Pharm, Key Lab Tibet Plateau Phytochem Qinghai Prov, Xining 810007, Peoples R China

第一机构：China West Normal Univ, Coll Chem & Chem Engn, Chem Synth & Pollut Control Key Lab Sichuan Prov, Shida Rd 1, Nanchong 637009, Peoples R China

通信机构：[1]corresponding author), China West Normal Univ, Coll Chem & Chem Engn, Chem Synth & Pollut Control Key Lab Sichuan Prov, Shida Rd 1, Nanchong 637009, Peoples R China;[2]corresponding author), Gansu Univ Chinese Med, Sch Clin Med 1, Lanzhou 730030, Gansu, Peoples R China;[3]corresponding author), Qinghai Nationalities Univ, Coll Pharm, Key Lab Tibet Plateau Phytochem Qinghai Prov, Xining 810007, Peoples R China.|[10735]甘肃中医药大学;

年份：2025

卷号：1321

外文期刊名：JOURNAL OF MOLECULAR STRUCTURE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001324642400001)】；

基金：This work was supported by the National Natural Science Foundation of China (NO. 22206154, 82303568) , the project funds of Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province (No. CSPC202401) , the Gansu Provincial Natural Science Foundation (NO. 23JRRA1216) and the Gansu Provincial University Innovation Foundation (No. 2023QB-085) .

语种：英文

外文关键词：Tripeptide; Fluorescent probe; Cd(II); Bio-imaging; Smartphone-assisted recognition

摘要：Cadmium ions (Cd2+) is a ubiquitous environmental pollutant that endangers all forms of life, and it has become critical to develop simple and highly selective detection methods for monitoring Cd2+. Herein, a new fluorescent turn-on probe DPGC with dansyl fluorophore labelled tripeptide (ProGlyCys-NH2) was successfully synthesized using solid-phase peptide synthesis technology with Fmoc chemistry. Interestingly, DPGC showed high specificity and fluorescence "turn on" response towards Cd2+ in 100% aqueous solution. In addition, the limit of detection (LOD) for Cd2+ recognition by DPGC was calculated to be 12.4 nM. The pH test showed that DPGC had a wide detection range of 7-11 for Cd2+, and the response time was also less than 30 s. Notably, the DPGC-Cd2+ complex forms a 2:1 binding stoichiometry was determined using fluorescence titration, Job's plot, ESI-HRMS and DFT calculations. Additionally, the binding of DPGC with Cd2+ was found fluorescence reversible on addition of EDTA. Meanwhile, DPGC has excellent membrane permeability and low cytotoxicity, and was successfully employed for detecting Cd(2+)in living cells and zebrafish. More importantly, the colour change of Cd2+ solution with different concentrations under 365 nm UV light was detected by a smartphone App to achieve semi-quantitative visual analysis of Cd2+ ions.