文献类型：期刊文献

中文题名：宣肺化浊方治疗新型冠状病毒感染肺炎的网络药理学分析

英文题名：Pharmacological Analysis of Xuanfei Huazhuo Prescription in Treatment of Coronavirus Disease-2019

作者：刘东玲[1];王浩嘉[2];任伟钰[1];苏敬[1];郑宜鋆[1];侯雯倩[1];靳晓杰[1];宁艳梅[1];李佳蔚[1];张志明[3];刘永琦[1,3]

第一作者：刘东玲

机构：[1]甘肃中医药大学,甘肃省高校重大疾病分子医学与中医药防治研究重点实验室教育部敦煌医学与转化重点实验室,兰州730000;[2]兰州大学第一临床医学院,兰州730000;[3]甘肃中医药大学附属医院,兰州730000

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2020

卷号：26

期号：16

起止页码：40

中文期刊名：中国实验方剂学杂志

外文期刊名：Chinese Journal of Experimental Traditional Medical Formulae

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：甘肃中医药大学新型冠状病毒感染的肺炎应急防治专项(2020XNYJ-05);甘肃省新型冠状病毒(2019-nCoV)肺炎应急科研专项;兰州市城关区科技计划项目(2020JSCX0025)。

语种：中文

中文关键词：新型冠状病毒肺炎;宣肺化浊方;生物信息学;多脏器损伤;细胞炎症因子风暴

外文关键词：coronavirus disease-2019(COVID-19);Xuanfei Huazhuo prescription;bioinformatics;multiple organ injury;cytokine storm

摘要：目的:通过网络药理学以及现代药理学基础分析探讨宣肺化浊方治疗新型冠状病毒肺炎(COVID-19)的作用靶点及信号通路,阐述其可能的作用机制。方法:从TCMSP和BATMAN-TCM数据库搜索宣肺化浊方各中药的化合物及靶点,使用GeneCards,NCBI以及CTD数据库检索COVID-19靶点;通过STRING数据库构建蛋白质相互作用网络。采用Cytoscape3. 7. 0软件构建中药-归经网络图以及中药-化合物-关键靶点-疾病网络,利用Omicshare平台对共有靶点进行京都基因与基因组百科全书(KEGG)分析及基因本体(GO)分析。除此之外,检索多脏器损伤、免疫损伤以及重症急性呼吸综合征(SARS)的疾病靶点,将宣肺化浊方与之做映射,计算交集靶点占宣肺化浊方靶点的比例。结果:宣肺化浊方共10味中药,其中有6味归肺经,5味归脾经,5味归胃经。化合物共409个,相对应的靶标2 271个。宣肺化浊方的靶点与新冠肺炎有8个相同的炎症因子,每个炎症因子对应多个化合物。宣肺化浊方与COVID-19有135个交集靶点,筛选得到36个关键靶点。KEGG通信号通路富集筛选得到172条信号通路(P<0. 05),GO分析得到生物过程结果有4 000个,细胞组成有254个,分子功能有408个(P<0. 05)。宣肺化浊方与各脏器损伤靶点和免疫损伤靶点的共有靶点较多,且共有靶点/XFHZP靶点的比例在7. 6%~97. 8%,宣肺化浊方与SARS有173个交集靶点。结论:宣肺化浊方可能通过发挥抗炎、保护脏器损伤、免疫等作用治疗新冠肺炎,这将为治疗新冠肺炎药物的开发提供一定的理论依据。
Objective:The targets and signaling pathways of Xuanfei Huazhuo prescription(XFHZP) for the treatment of coronavirus disease-2019(COVID-19)were explored,and its possible action mechanisms were described through network pharmacology and basic analysis of modern pharmacology. Method: The compounds and targets in XFHZP were collected through TCMSP and BATMAN-TCM databases. The targets of COVID-19 were studied by GeneCards,NCBI and CTD databases. The PPI network was constructed through STRING database. The networks of "herb-meridian" and "traditional Chinese medicines-compounds-targetsdisease" were generated by Cytoscape 3. 7. 0. Then,Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis and Gene Ontology(GO)analysis were made for shared targets through the Omicshare platform. In addition,the disease targets of multiple organ injury,immune injury and severe acute respiratory syndrome(SARS)were retrieved and then mapped with XFHZP. The ratio of intersection targets to XFHZP’s targets was calculated. Result: XFHZP has 10 traditional Chinese medicines in total,including 6 medicines with the meridian tropism to lung,5 medicines with the meridian tropism to the spleen and 5 medicines with the meridian tropism to the stomach. There were 409 compounds and 2 271 targets. There were 8 same inflammatory factors in targets between XFHZP and COVID-19,and each inflammatory factor corresponded to multiple compounds.XFHZP and COVID-19 had 135 intersection targets,and 36 key targets were screened out. A total of 172 signaling pathways were screened out through KEGG signal pathway enrichment(P<0. 05). There were 4 000 biological processes,254 cell components,and 408 molecular functions(P<0. 05)according to GO analysis.XFHZP had many common targets with various organ damage targets and immune damage targets,with the ratio of about 7. 6%-97. 8%. XFHZP had 173 intersection targets with SARS. Conclusion: XFHZP may treat COVID-19 through anti-inflammatory,organ protecting and immune effects. It will provide a certain theoretical basis for the development of drugs for COVID-19.