文献类型：期刊文献

中文题名：三黄凝胶调控TLRs/NLRP3信号通路改善大鼠痤疮模型的作用机制

英文题名：Mechanism of Sanhuang Gel regulating TLRs/NLRP3 signaling pathway to improve acne model in rats

作者：杜雪洋[1];牛凡琪[1];董小平[1];杨鹏斐[1];周文丽[2];王思农[1]

第一作者：杜雪洋

机构：[1]甘肃中医药大学,甘肃兰州730000;[2]甘肃中医药大学附属医院,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2025

卷号：30

期号：6

起止页码：741

中文期刊名：中国临床药理学与治疗学

外文期刊名：Chinese Journal of Clinical Pharmacology and Therapeutics

收录：;北大核心:【北大核心2023】；

基金：甘肃省高等学校科技成果转化项目(2017D-17);国家重点研发计划“中医药现代化研究”重点专项(2018YFC1705302);甘肃省高校产业支撑计划项目(2025CYZC-046)。

语种：中文

中文关键词：三黄凝胶;痤疮模型;TLRs/NLRP3信号通路

外文关键词：Sanhuang Gel;acne model;TLRs/NL-RP3 signaling pathway

摘要：目的:研究三黄凝胶对耳廓痤疮模型大鼠TLRs/NLRP3信号通路表达的影响,探讨其治疗痤疮炎症损伤的作用机制。方法:采用右耳导管开口处涂抹100%油酸+痤疮丙酸杆菌注射诱导大鼠耳廓痤疮模型,大鼠分为正常组、模型组、阳性对照组(盐酸克林霉素凝胶10 mg/g)、三黄凝胶高、中、低剂量组(280、140、70 mg/g)。各给药组分别给予药物干预28 d。观察各组大鼠耳廓皮损变化;苏木素-伊红(HE)染色观察耳廓痤疮病理形态改变;透射电镜(TEM)观察耳廓组织细胞超微结构改变;免疫荧光法检测大鼠耳廓组织中TLR2和TLR4分布和表达变化;采用实时荧光定量聚合酶链式反应(Real-time PCR)测定大鼠耳廓组织TLR2、TLR4、NLRP3、ASC和Caspase-1 mRNA的表达;蛋白免疫印迹法(Western blot)测定大鼠耳廓组织TLR2、TLR4、NLRP3、ASC和Caspase-1蛋白的表达。结果:与正常组比较,模型组耳廓组织可见丘疹、脓疱,炎性细胞浸润,线粒体明显水肿;TLR2、TLR4、NLRP3、ASC、Caspase-1 mRNA和蛋白表达水平明显升高(P<0.01)。与模型组比较,各给药组耳廓外观和病理损伤明显减轻,线粒体形态以及结构恢复正常;TLR2、TLR4、NLRP3、ASC和Caspase-1 mRNA表达水平明显降低(P<0.01)。阳性对照组和高、中剂量组TLR2、TLR4、NLRP3、ASC和Caspase-1蛋白表达水平降低(P<0.01、P<0.05);低剂量组ASC和Caspase-1蛋白表达水平明显降低(P<0.01),TLR2、TLR4、NLRP3蛋白表达水平无明显差异(P>0.05)。结论:三黄凝胶能改善痤疮大鼠耳廓组织病理损伤,降低免疫炎症反应,其作用机制可能与调控TLRs/NLRP3信号通路相关。
AIM:To study the effect of Sanhuang Gel on the expression of TLRs/NLRP3 signal pathway in auricle acne model rats,and to explore its mechanism in treating acne inflammatory injury.METHODS:A rat model of auricular acne was induced by applying 100%oleic acid to the opening of the right ear catheter and injecting Propionibacterium acnes.Rats were divided into normal group,model group,positive control group(clindamycin hydrochloride gel 10 mg/g)and Sanhuang Gel high,medium and low dose groups(280,140 and 70 mg/g).Each drug group was given drug intervention for 28 days.The changes of auricle skin lesions in each group were observed;Hematoxylin-eosin(HE)staining was used to observe the pathological changes of auricle acne.The ultrastructural changes of auricle tissue cells were observed by transmission electron microscope(TEM).The distribution and expression changes of TLR2 and TLR4 in rat auricle tissue were detected by immunofluorescence.The expressions of TLR2,TLR4,NLRP3,ASC and Caspase-1 mRNA in rat auricle were detected by Real-time PCR.The expressions of TLR2,TLR4,NLRP3,ASC and Caspase-1 in rat auricle were detected by Western blot.RESULTS:Compared with the normal group,papules,pustules,inflammatory cell infiltration and obvious edema of mitochondria can be seen in the auricle tissue of the model group.The mRNA and protein expression levels of TLR2,TLR4,NLRP3,ASC andCaspase-1 increased significantly(P<0.01).Compared with the model group,the appearance and pathological damage of auricle in each treatment group were obviously alleviated,and the morphology and structure of mitochondria returned to normal.The mRNA expression levels of TLR2,TLR4,NLRP3,ASC and Caspase-1 decreased significantly(P<0.01).The expression levels of TLR2,TLR4,NLRP3,ASC and Caspase-1 in positive control group and high and middle dose groups decreased(P<0.01,P<0.05).The expression levels of ASC and Caspase-1 protein in low dose group were significantly decreased(P<0.01),but there was no significant difference in the expression levels of TLR2,TLR4 and NLRP3 protein(P>0.05).CONCLUSION:Sanhuang Gel can improve the pathological damage of auricle tissue and reduce immune inflammatory reaction in acne rats,and its mechanism may be related to regulating TLRs/NLRP3 signaling pathway.