文献类型：期刊文献

中文题名：艰难梭菌毒素A诱导K562细胞凋亡及其机制研究

英文题名：Apoptosis-inducing Effect of Clostridium Difficile Toxin A on K562 Cells and Its Mechanism

作者：李培[1];陈彻[2];席亚明[1];张豪[1];李明[1];邓伟[1]

第一作者：李培

机构：[1]兰州大学第一医院血液科,血液学研究所,甘肃兰州730000;[2]甘肃中医学院科研中心,甘肃兰州730000

第一机构：兰州大学第一医院血液科,血液学研究所,甘肃兰州730000

年份：2011

卷号：19

期号：3

起止页码：638

中文期刊名：中国实验血液学杂志

外文期刊名：Journal of Experimental Hematology

收录：CSTPCD;;Scopus;CSCD:【CSCD_E2011_2012】；PubMed;

语种：中文

中文关键词：白血病;K562细胞;凋亡;艰难梭菌毒素A

外文关键词：leukemia; K562 cell; apoptosis; Clostridium difficile toxin A

摘要：本研究探索艰难梭菌毒素A(Tcd A)对人慢性髓系白血病细胞株K562细胞增殖和凋亡的影响及作用机制。采用MTT法检测Tcd A的细胞毒作用,用Hoechst33342染色和流式细胞术观察细胞凋亡,免疫细胞化学法检测BCL-2、BAX蛋白表达水平,比色法检测caspase-3活性。结果表明,Tcd A能明显抑制K562细胞增殖,具有浓度和时间依赖性;荧光染色和流式细胞术检测到细胞凋亡;Tcd A作用后BCL-2蛋白表达下降,而BAX蛋白表达明显增加,与对照组相比差异有统计学意义(p<0.05);caspase-3活性增强,差异有统计学意义(p<0.05)。结论 :Tcd A能明显抑制K562细胞增殖,并诱导其凋亡,其机制可能与上调BAX蛋白表达,激活caspase-3有关。
This study was purposed to investigate the growth inhibition and apoptosis-inducing effect of Clostridium difficile toxin A（Tcd A） on the leukemia cell line K562. The proliferative activity of K562 cells exposed to Ted A was tested by MTT assay, cell apoptosis was detected by flow cytometry; immunocytochemistry and colorimetric assay were employed to detect the protein expressions of BCL-2/BAX and the activity of Caspase-3, respectively. The results indicated that the proliferation of K562 ceils was inhibited in a time-and dose-dependent manner after exposure to Ted A for 24,48 and 72 hours, the ceils displayed the typical apoptotic, morphological changes, the expression of BCL-2 protein was down-regulated but the expression of BAX protein was signicantly increased, compared with control group（p 〈 0.05 ）. In addition, caspase-3 was activated in a concentration-dependent manner. It is concluded that Ted A inhibits cell growth of K562 by inducing apoptosis, and the up-regulation of BAX protein and activation of caspase-3 may play important roles in these processes.