文献类型：期刊文献

中文题名：黄芪多糖对电离辐射骨髓间充质干细胞向神经分化潜能的影响

英文题名：Effects of Astragalus polysaccharide on neuronal differentiation potential of mesenchymal stem cell induced by ionizing radiation

作者：卢志伟[1];王磊[1];张利英[1];何进鹏[2];丁楠[2];任春贞[1];李玲[1];王凤梅[1];刘永琦[1,3]

第一作者：卢志伟

机构：[1]甘肃中医药大学基础医学院,甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室;[2]甘肃省空间辐射生物学重点实验室,中国科学院近代物理研究所;[3]敦煌医学与转化省部共建教育部重点实验室

第一机构：甘肃中医药大学基础医学院（敦煌医学研究所）|甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2017

卷号：33

期号：7

起止页码：950

中文期刊名：中国药理学通报

外文期刊名：Chinese Pharmacological Bulletin

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2017_2018】；

基金：国家自然科学基金资助项目(No 81473457);甘肃中医药大学中青年项目(No ZQ2014-11)

语种：中文

中文关键词：黄芪多糖;电离辐射;骨髓间充质干细胞;神经分化潜能;尼氏体;保护作用

外文关键词：Astragalus polysaccharide; ionizing radiation; bone marrow mesenchymal stem cells; neuronal differentiation potential; Nissl body; protective effect;

摘要：目的研究黄芪多糖对X射线照射人骨髓间充质干细胞向神经分化的干预作用。方法采用CCK-8法筛选不同浓度的黄芪多糖作用于2Gy X射线辐射人骨髓间充质干细胞的增殖能力;细胞形态计数X射线对骨髓间充质干细胞分化为神经细胞数量的影响;甲苯胺蓝染色检测X射线对骨髓间充质干细胞分化为神经细胞中尼氏体的表达量;Western blot检测神经巢蛋白(Nestin)及神经元特异性烯醇化酶(neuron-specific enolase,NSE)表达变化。结果与对照组相比,X射线照射后细胞增殖水平明显降低(P<0.05),与单纯照射组比较,加药照射组骨髓间充质干细胞增殖水平显著增加(P<0.05),其中黄芪多糖浓度为50 mg·L^(-1)时促增殖作用最强,设为最佳药物浓度;单纯辐射后骨髓间充质干细胞分化为神经细胞的数量明显降低(P<0.05),当给予黄芪多糖干预后分化为神经细胞的数量明显上升(P<0.05);辐射后骨髓间充质干细胞分化为神经细胞中尼氏体的表达量降低(P<0.05),当给予黄芪多糖干预后,尼氏体的表达量明显增多;单纯辐射后Nestin及NSE表达量降低(P<0.05),而当给予黄芪多糖干预后,Nestin和NSE表达量升高(P<0.05)。结论黄芪多糖对电离辐射人骨髓间充质干细胞向神经分化潜能的影响具有保护作用。
Aim To investigate the effect of Astragalus polysaccharide（APS） on the differentiation ability of human bone marrow mesenchymal stem cells（bone marrow mesenchymal stem cells,BMSCs） into nerve cells after irradiated by X rays.Methods We used different concentrations of APS to treat BMSCs which were exposed in 2 Gy X ray radiation,then we selected the proliferation ability of BMSCs by CCK-8 method.We also counted the nerve cells that were differentiated from BMSCs.Then we detected the expression of Nissl bodies by Toluidine blue staining,and we also tested the expression of Nestin and NSE by Western blot.Results Compared with the control group,the ability of cell proliferation significantly decreased after X ray radiation（P〈0.05）,compared with radiation group,the proliferation ability of BMSCs treated by APS significantly increased（P〈0.05）.Moreover,BMSCs had the best proliferation ability which was treated by APS in the concentration of 50 mg · L-1.Then we chose this dose as the best drug concentration.The number of nerve cells significantly decreased after radiation（P〈0.05）.Neural cells significantly increased（P〈0.05） after treated by APS.We found the expression of Nissl bodies decreased after radiation（P〈0.05）,and increased significantly（P〈0.05） after treated by APS.The expression of Nestin and NSE decreased after radiation（P〈0.05）,and their expression level increased after treated by APS（P〈0.05）.Conclusion APS has protective effects on the differentiation potential of human bone marrow mesenchymal stem cells into nerve cells exposed in ionizing radiation.