文献类型：期刊文献

英文题名：Establishment and characterization of DPC-X4: a novel mixed-type ampullary cancer cell line

作者：Chai, Changpeng[1,2];Tang, Huan[2];Yi, Jianfeng[3,4];Li, Lu[1];Yu, Cheng[2,5];Su, Yuanhui[2];Miao, Long[1,2];Ye, Zhenzhen[2,6];Wang, Zhengfeng[1,2];Luo, Wei[1];Hu, Jinjing[1];Zhang, Hui[2,7];Miao, Xin[8];Xu, Hao[1,3];Zhou, Wence[2,7]

第一作者：Chai, Changpeng

通信作者：Xu, H[1];Zhou, WC[2];Xu, H[3];Zhou, WC[4];Miao, X[5]

机构：[1]Lanzhou Univ, Hosp 1, Dept Gen Surg 4, Lanzhou 730000, Peoples R China;[2]Lanzhou Univ, Clin Med Coll 2, Lanzhou 730000, Peoples R China;[3]Lanzhou Univ, Clin Med Coll 1, Lanzhou 730000, Peoples R China;[4]Gansu Univ Chinese Med, Sch Clin Med 1, Dept Surg, Lanzhou 730000, Peoples R China;[5]Lanzhou Univ, Hosp 2, Dept Anesthesiol, Lanzhou 730000, Peoples R China;[6]Gansu Univ Chinese Med, Sch Clin Med 1, Lanzhou, Gansu, Peoples R China;[7]Lanzhou Univ, Hosp 2, Dept Gen Surg, 82 Cuiyingmen, Lanzhou 730000, Peoples R China;[8]Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Key Lab Anim Virol,Minist Agr, Lanzhou 730046, Peoples R China

第一机构：Lanzhou Univ, Hosp 1, Dept Gen Surg 4, Lanzhou 730000, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Hosp 1, Dept Gen Surg 4, Lanzhou 730000, Peoples R China;[2]corresponding author), Lanzhou Univ, Clin Med Coll 2, Lanzhou 730000, Peoples R China;[3]corresponding author), Lanzhou Univ, Clin Med Coll 1, Lanzhou 730000, Peoples R China;[4]corresponding author), Lanzhou Univ, Hosp 2, Dept Gen Surg, 82 Cuiyingmen, Lanzhou 730000, Peoples R China;[5]corresponding author), Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Key Lab Anim Virol,Minist Agr, Lanzhou 730046, Peoples R China.

年份：2024

卷号：37

期号：2

起止页码：531

外文期刊名：HUMAN CELL

收录：;Scopus(收录号：2-s2.0-85182850099);WOS:【SCI-EXPANDED(收录号:WOS:001151398900001)】；

基金：We would like to thank Bullet Edits (http://www.bulletedits.cn) for English language editing of the manuscript.

语种：英文

外文关键词：Mixed-type ampullary cancer; DPC-X4 cell line; Drug sensitivity; Xenograft tumor model; Pathogenetic research

摘要：Mixed-type ampullary cancer is a distinct subtype of ampullary cancer that manifests a merging of the biological characteristics of both intestinal and pancreaticobiliary subtypes. The absence of established cell lines specific to this subtype has resulted in a concomitant scarcity of research on its tumorigenic mechanisms and the development of novel therapeutic modalities. The present study achieved the successful establishment of a novel mixed-type ampullary cancer cell line, designated DPC-X4 through primary culture techniques. Subsequent analyses pertaining to phenotypic characteristics, molecular profiling, biomarker identification, and histological features validated the DPC-X4 cell line as a potent model for delineating the pathogenesis of mixed-type ampullary cancer and facilitating the development of new pharmacological agents. This newly established cell line was subjected to continuous cultivation for 1 year, with stable passaging for over 50 generations. Notably, the DPC-X4 cell line manifested typical morphological features associated with epithelial tumors. Furthermore, the population doubling time for the DPC-X4 cell line was determined at 70 h. Short tandem repeat (STR) analysis confirmed that the DPC-X4 cell line exhibited a high genetic concordance with the primary tumor from the patient. Karyotypic profiling indicated an abnormal sub-triploid karyotype, with representative karyotypes of 57, XXY inv (9), 14p + , 15p + , der (17), + mar. The DPC-X4 cell line demonstrated a high capacity for efficient organoid formation under suspension culture conditions. In addition, the subcutaneous inoculation of DPC-X4 cells into NXG mice led to the formation of xenografted tumors. The results of drug sensitivity testing indicated that DPC-X4 cells were sensitive to paclitaxel and resistant to oxaliplatin, 5-fluorouracil, and gemcitabine. Immunohistochemistry revealed positive expression of CK7, CK19, and CK20 in DPC-X4 cells, while CDX2 demonstrated negative expression. In addition, positive expression of E-cadherin and vimentin was identified in DPC-X4 cells, with a proliferation index indicated by Ki-67 at 70%. The findings of our study establish DPC-X4 as a novel mixed-type ampullary cancer cell line, which can serve as a potential experimental model for exploring the pathogenesis of ampullary cancer and the development of therapeutic drugs.